{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T02:21:08Z","timestamp":1776824468618,"version":"3.51.2"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,6,27]],"date-time":"2011-06-27T00:00:00Z","timestamp":1309132800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The CINtec PLUS\u00ae system is an immunohistochemical cocktail composed of antibodies against p16<jats:sup>INK4a<\/jats:sup> (surrogate of HPV infection) and Ki\u201067 (proliferation marker) meant to improve the sensitivity and specificity for detecting high\u2010grade dysplasia (HGD). In the presence of dysplasia, a red chromogen marks Ki\u201067 expression in the nucleus and a brown chromogen marks cytoplasmic p16<jats:sup>INK4a<\/jats:sup> expression. Only cells showing dual staining are interpreted as positive. This retrospective study examined the performance of CINtec PLUS testing when performed on ASC\u2010US diagnosed samples. Comparison was made to high\u2010risk HPV DNA test results and colposcopic biopsy results. Technical considerations in the interpretation of this immunohistochemical stain are additionally discussed. CINtec PLUS showed modest sensitivity (64%) and specificity (53%) in identifying the presence of HGD at surgical biopsy. Positive and negative predictive values for HGD were 28% and 83%, respectively. HR\u2010HPV DNA test yielded sensitivity of 100% and specificity of 21%. During interpretation, squamous metaplasia and endocervical cells were seen to show individual staining for p16<jats:sup>INK4a<\/jats:sup> or Ki\u201067. Individual staining, when present within three dimensional cellular groups common to SurePath\u00ae preparations, can be time\u2010intensive to interpret necessitating thoughtful examination at high power. The Pap test with HR\u2010HPV DNA testing is a highly sensitive test. A specific test is needed to prevent false positives and over treatment. The CINtec\u00ae system provides a modest increase in specificity beyond HR\u2010HPV DNA testing. Future study of its appropriateness and cost\u2010ffectiveness in a treatment algorithm are warranted. Diagn. Cytopathol. 2013. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.21757","type":"journal-article","created":{"date-parts":[[2011,6,30]],"date-time":"2011-06-30T12:39:00Z","timestamp":1309437540000},"page":"35-40","source":"Crossref","is-referenced-by-count":25,"title":["Evaluation of CINtec PLUS\u00ae testing as an adjunctive test in ASC\u2010US diagnosed SurePath\u00ae preparations"],"prefix":"10.1002","volume":"41","author":[{"given":"Neil","family":"Edgerton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Momin T.","family":"Siddiqui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,6,27]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.16.2114"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2007.07.047"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.24760"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.2009.164350"},{"key":"e_1_2_5_6_2","first-page":"1","article-title":"Surveillance of screening\u2010detected cancers (colon and rectum, breast, and cervix)\u2014United States, 2004\u20132006","volume":"59","author":"Henley SJ","year":"2010","journal-title":"MMWR Surveill Summ"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.11.1500"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/24.2.300"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/315086"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199802123380703"},{"key":"e_1_2_5_11_2","first-page":"609","article-title":"Behavior of moderate cervical dysplasia during long\u2010term follow\u2010up","volume":"61","author":"Nasiell K","year":"1983","journal-title":"Obstet Gynecol"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204869"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.63.10.4417-4421.1989"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.9.4350"},{"key":"e_1_2_5_15_2","first-page":"629","article-title":"New Ki\u201067\u2010equivalent murine monoclonal antibodies (MIB 1\u20103) generated against bacterially expressed parts of the Ki\u201067 cDNA containing three 62 base pair repetitive elements encoding for the Ki\u201067 epitope","volume":"68","author":"Key G","year":"1993","journal-title":"Lab Invest"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2010.06.011"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2010.078634"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPXSVCDZ3D5MZM"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22420"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2008.10.005"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP3CD9YKYFJDQL"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-08-0508"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(08)70208-0"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22580"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21378"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djj009"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdc.21757","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.21757","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T18:58:54Z","timestamp":1697482734000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.21757"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6,27]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1002\/dc.21757"],"URL":"https:\/\/doi.org\/10.1002\/dc.21757","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,6,27]]}}}