{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T13:20:52Z","timestamp":1783948852707,"version":"3.55.0"},"reference-count":21,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,7,26]],"date-time":"2012-07-26T00:00:00Z","timestamp":1343260800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>ASC and LSIL comprise the majority of abnormal Pap smears. Currently, high\u2010risk human papillomavirus testing is utilized to triage women with ASC for colposcopy; however, no cost effective triage method is available for LSIL. p16 and Ki\u201067 have each been shown to be good biomarkers for high grade cervical intraepithelial neoplasia (HG CIN).We evaluated the role of the CINtec\u00ae PLUS p16\/Ki\u201067 dual immunostain as a marker for underlying (U) or subsequent (S) HG CIN. One hundred and eighty eight cervical SurePath Pap smears with histological and\/or cytological follow\u2010up were retrieved from our departmental files. The Pap stained slides were destained and then immunostained utilizing the CINtec\u00ae PLUS dual staining reagent kit. Results of the dual stain were correlated with follow\u2010up diagnoses. Sensitivity, specificity, and positive and negative predictive values of CINtec\u00ae PLUS for U or S HG CIN were compared with those of HR HPV testing and with p16 and Ki\u201067 immunostaining alone. The sensitivity of CINtec\u00ae PLUS for U or S HG CIN was 91% in the ASC group and 100% in the LSIL group, while the corresponding specificities were 61 and 43%, respectively. The sensitivity and specificity of CINtec\u00ae PLUS for U or S HG CIN in both groups combined were 97 and 53%, respectively. CINtec\u00ae PLUS was more specific than HR HPV testing and Ki\u201067 and p16 immunostains alone in detecting an U or S HG CIN. CINtec\u00ae PLUS is a helpful adjunct in identifying U or S HG CIN when applied to SurePath Pap smears with ASC or LSIL. Diagn. Cytopathol. 2013. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.22900","type":"journal-article","created":{"date-parts":[[2012,7,26]],"date-time":"2012-07-26T08:21:32Z","timestamp":1343290892000},"page":"582-587","source":"Crossref","is-referenced-by-count":36,"title":["CINtec\u00ae PLUS dual immunostain: A triage tool for cervical pap smears with atypical squamous cells of undetermined significance and low grade squamous intraepithelial lesion"],"prefix":"10.1002","volume":"41","author":[{"given":"Sanam","family":"Loghavi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann E.","family":"Walts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shikha","family":"Bose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,7,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.5858\/2004-128-1224-BIARRP","article-title":"Bethesda 2001 implementation and reporting rates: 2003 practices of participants in the College of American pathologists interlaboratory comparison program in cervicovaginal cytology","volume":"128","author":"Davey DD","year":"2004","journal-title":"Archives of pathology and laboratory medicine"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2007.07.047"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2003.461"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000207557.30226.25"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djj009"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-08-0306"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1155\/2007\/678793"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP3CD9YKYFJDQL"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1309\/0DGGQBDQAMJCJBXB"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22284"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22580"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20822"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-08-0508"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20046"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(02)00462-8"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.3802\/jgo.2008.19.3.162"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20140"},{"key":"e_1_2_6_19_2","volume-title":"HPV HR [Package Insert]","author":"Cervista TM","year":"2009"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.2307\/2531595"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20188"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2011.02.033"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdc.22900","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.22900","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T10:45:59Z","timestamp":1700649959000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.22900"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,26]]},"references-count":21,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1002\/dc.22900"],"URL":"https:\/\/doi.org\/10.1002\/dc.22900","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,26]]}}}