{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T13:46:36Z","timestamp":1770471996718,"version":"3.49.0"},"reference-count":50,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,1,22]],"date-time":"2013-01-22T00:00:00Z","timestamp":1358812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>The clinical usefulness of the ProEx C (Becton Dickinson) and PreTect HPV\u2010Proofer E6\/E7 mRNA tests (Proofer; Norchip) for the triage of ASCUS and LSIL cytology was determined in comparison with the Hybrid Capture 2 HPV DNA test (HC2; Qiagen). The study population consisted of women with a history of abnormal cytology referred to colposcopy. Histology\u2010confirmed CIN 2+ served as the disease endpoint. The study was based on 1,360 women (mean age 30.7 years), of whom 380 had CIN 2+. Among 315 with ASCUS (CIN 2+, n = 67), the sensitivities of ProEx C, Proofer, and HC2 to detect CIN 2+ were, 71.6, 71.6, and 95.5%, respectively, with a corresponding specificity of 74.6, 74.2, and 35.1%. Among 363 with LSIL (CIN 2+, n = 108), the sensitivities of ProEx C, Proofer, and HC2 were, 67.6, 74.1, and 96.3%, respectively, with a corresponding specificity of 60, 68.2, and 18.4%. Among 225 HC2\u2010positive ASCUS (CIN 2+, n = 64), 105 tested positive by ProEx C, reducing colposcopy referral by 53.3% and detecting 71.9% of CIN 2+; Proofer was positive in 112\/225, reducing colposcopy referral by 50.2% and detecting 75.0% of CIN 2+. Among 312 HC2\u2010positive LSIL (CIN 2+, n = 104), 160 tested positive by ProEx C, reducing coloposcopy referral by 48.7% and detecting 66.3% of CIN 2+; Proofer was positive in 159\/312, reducing colposcopy referral by 49.0% and detecting 75.0% of CIN 2+. In conclusion, both ProEx C and Proofer have a similar performance profile with a significantly higher specificity but lower sensitivity than HC2 for the detection of CIN 2+. Consequently, although they can reduce colposcopy referral, they will miss a proportion of CIN 2+ cases. This is a major limitation and should be taken into account if these tests are considered for ASCUS or LSIL triage. Diagn. Cytopathol. 2013;41:767\u2013775. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.22944","type":"journal-article","created":{"date-parts":[[2013,1,22]],"date-time":"2013-01-22T15:46:15Z","timestamp":1358869575000},"page":"767-775","source":"Crossref","is-referenced-by-count":22,"title":["Performance of proex c and pretect hpv\u2010proofer e6\/e7 mrna tests in comparison with the hybrid capture 2 hpv dna test for triaging ascus and lsil cytology"],"prefix":"10.1002","volume":"41","author":[{"given":"Reza","family":"Alaghehbandan","sequence":"first","affiliation":[{"name":"Department of Anatomic Pathology Faculty of Medicine Memorial University St. John's Newfoundland and Labrador Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Fontaine","sequence":"additional","affiliation":[{"name":"Department of Anatomical Pathology Faculty of Medicine University of Calgary Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Bentley","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Queen Elizabeth II Health Sciences Centre Halifax Nova Scotia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas","family":"Escott","sequence":"additional","affiliation":[{"name":"Department of Pathology Regional Health Sciences Centre Thunder Bay Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prafull","family":"Ghatage","sequence":"additional","affiliation":[{"name":"Department of Gynecology Tom Baker Cancer Centre Calgary Alberta Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian","family":"Lear","sequence":"additional","affiliation":[{"name":"Department of Oncology Dr. Bliss H. 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