{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T13:24:51Z","timestamp":1725542691092},"reference-count":24,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T00:00:00Z","timestamp":1367280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>Herein, we evaluated cervical samples from normal tissue or HPV\u2010infected tissue, to determine if the relative nuclear\/cytoplasmic ratio (NA\/CA) and the presence of nonclassical cytological criteria are a novel cytological criterion for the diagnosis of HPV. Significantly, larger NA\/CA ratios were found for the HPV\u2212ATYPIA+ and HPV+ATYPIA+ groups compared with HPV\u2212ATYPIA\u2212 group, regardless of collection method. For the samples collected with a spatula, only three samples from the HPV\u2212ATIPIA\u2212 group showed four or more nonclassical parameters (i.e., were positive), while a larger number of the samples in the HPV\u2212ATYPIA+, HPV+ATYPIA\u2212, and HPV+ATYPIA+ groups were positive (13, 4, and 13 samples, respectively). Among those collected with a brush, no sample showed four or more nonclassical criteria in the HPV\u2212ATYPIA\u2212 group, while a number of samples were positive in the HPV\u2212ATYPIA+, HPV+ATYPIA\u2212, and HPV+ATYPIA+ groups (4, 3, and 4 samples, respectively). HPV infection was associated with significant morphometrical changes; no increase in the NA\/CA ratio was found in the HPV+ATYPIA\u2010 samples, compared with the HPV\u2212ATIPIA\u2212 samples collected with either a spatula or a brush. In conclusion, by including nonclassical cytological criteria into the patient diagnosis, we were able to reduce the number of false negative and false positive HPV diagnoses made using conventional cytology alone. Diagn. Cytopathol. 2013;41:785\u2013792. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.22955","type":"journal-article","created":{"date-parts":[[2013,4,30]],"date-time":"2013-04-30T09:13:04Z","timestamp":1367313184000},"page":"785-792","source":"Crossref","is-referenced-by-count":2,"title":["Morphometric evaluation and nonclassical criteria for the diagnosis of HPV infection and cytological atypia in cervical samples"],"prefix":"10.1002","volume":"41","author":[{"given":"Dandara Emery Morais","family":"Sana","sequence":"first","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas Escola de Farm\u00e1cia Universidade Federal de Ouro Preto (UFOP) Ouro Preto MG Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscila","family":"Mayrink de Miranda","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Instituto Butantan S\u00e3o Paulo SP 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Silva","sequence":"additional","affiliation":[{"name":"Departamento de Ginecologia Universidade Federal de S\u00e3o Paulo (UNIFESP) S\u00e3o Paulo SP Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"de C\u00e1ssia Stocco","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Instituto Butantan S\u00e3o Paulo SP Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willy","family":"Be\u00e7ak","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Instituto Butantan S\u00e3o Paulo SP Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ang\u00e9lica Alves","family":"Lima","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas Escola de Farm\u00e1cia Universidade Federal de Ouro Preto (UFOP) Ouro Preto MG Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia Martins","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas ICEB II Universidade Federal de Ouro Preto (UFOP) Ouro Preto MG Brasil"},{"name":"Laborat\u00f3rio de Pesquisas Cl\u00ednicas CiPharma (Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Farmac\u00eauticas) Escola de Farm\u00e1cia Universidade Federal de Ouro Preto (UFOP) Ouro Preto MG Brasil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,30]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000328290"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1309\/J086T2UB1WDL1LQU"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910450408"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"3514","DOI":"10.1128\/JCM.37.11.3514-3517.1999","article-title":"Analysis by 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