{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:09:31Z","timestamp":1722470971698},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,11,28]],"date-time":"2013-11-28T00:00:00Z","timestamp":1385596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:p>Current cervical screening uses a combination of cytology and high\u2010risk human papillomavirus (HR\u2010HPV) analysis in cases of atypical squamous cells of undetermined significance (ASCUS) and atypical squamous cells cannot exclude high\u2010grade intraepithelial lesion (ASC\u2010H). These diagnoses are subject to interobserver variability and HR\u2010HPV analysis can be limited by sampling inadequacy. This study correlates immunoexpression of P16 and Ki\u201067 in residual cervicovaginal material against cytology category and HR\u2010HPV status. Eighteen pap tests were selected: 8 ASCUS, 4 ASC\u2010H, and 6 controls (2 LSIL and 4 HSIL). Digene Hybrid Capture II test was used to detect HR\u2010HPV. The cytospins were stained for P16\/Ki\u201067. Pap tests, P16, Ki\u201067, HR\u2010HPV result and available biopsies were correlated. P16 expression correlated with HR\u2010HPV status in 15\/17 cases. Discordant cases (1 ASCUS and 1 ASC\u2010H) were +P16\/\u2013HR\u2010HPV. Ki\u201067 correlated with HR\u2010HPV in 8\/15 cases. Discordant cases were +HR\u2010HPV\/\u2013 Ki\u201067 (HSIL, LSIL, and ASC\u2010H one each), and \u2013HR\u2010HPV\/+Ki\u201067 (3 ASCUS, 1 LSIL, 1 ASC\u2010H). Two cases were\u2009+\u2009P16\/+ Ki\u201067\/\u2013 HR\u2010HPV. None were \u2010 P16\/\u2013 Ki\u201067\/+ HR\u2010HPV. Histologic follow\u2010up in 13 cases varied from benign to CIN III. Two cases of +P16\/ \u2013 Ki\u201067\/\u2013 HR\u2010HPV had benign cervical biopcies. Although a small sample size, our findings show a utility for adjunct P16\/ Ki\u201067 in addition to HR\u2010HPV testing in cases of squamous atypia when HR\u2010HPVs are non\u2010detected due to low DNA copies, or missed lesions in cervical biopsies. Diagn. Cytopathol. 2014;42:576\u2013581. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.23076","type":"journal-article","created":{"date-parts":[[2013,11,28]],"date-time":"2013-11-28T14:58:47Z","timestamp":1385650727000},"page":"576-581","source":"Crossref","is-referenced-by-count":6,"title":["Utility of P16 expression and Ki\u201067 proliferation index in ASCUS and ASC\u2010H pap tests"],"prefix":"10.1002","volume":"42","author":[{"given":"Adam D.","family":"Toll","sequence":"first","affiliation":[{"name":"Division of Cytopathology Department of Pathology The Johns Hopkins Hospital Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deidra","family":"Kelly","sequence":"additional","affiliation":[{"name":"Division of Cytopathology Department of Pathology The Johns Hopkins Hospital Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra","family":"Maleki","sequence":"additional","affiliation":[{"name":"Division of Cytopathology Department of Pathology The Johns Hopkins Hospital Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2004.00227.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199802123380703"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/85.12.958"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21513"},{"issue":"10","key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.5858\/arpa.LGT200570","article-title":"The Lower Anogenital Squamous Terminology Standardization Project for HPV\u2010Associated Lesions: Background and consensus recommendations from the College of American Pathologists and the American Society for Colposcopy and Cervical Pathology","volume":"136","author":"Darragh TM","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14955-0"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.981"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2001.1845"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(03)00418-6"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000331047"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.RCT.0000157092.44680.25"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22580"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000326711"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21349"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.1174"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.56.9.681"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20258"},{"key":"e_1_2_6_19_1","article-title":"The Bethesda System for Reporting Cervical Cytology, Definitions, Criteria, and Explanatory Notes","author":"Solomon D","year":"2003","journal-title":"Springer"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000325839"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2006.045948"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","unstructured":"ACOG Practice Bulletin.Clinical Management Guidelines for Obstetrician\u2010Gynecologists. 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