{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T05:40:56Z","timestamp":1696570856611},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,2,18]],"date-time":"2014-02-18T00:00:00Z","timestamp":1392681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>Aberrant expression of high mobility group box 1 (HMGB1) is associated with tumor development and progression. The current study was conducted to evaluate the significance of HMGB1 immunostaining on cell block (CB) preparations in the diagnosis of neoplastic and preneoplastic lesions of the cervix. The CBs were prepared from 157 residual liquid\u2010based gynecologic cytology specimens which were collected from women whose cervical lesions had been confirmed by histopathology. The expression of HMGB1 and p16INK4A (p16) was visualized by immunocytochemistry on the CB preparations, and the association of their expression patterns was correlated with the severity of cervical lesions. HeLa cells were used as positive control. HMGB1 expression was observed in dysplastic and neoplastic cells and increased along with the progression of cervical neoplasia. The rates of positive staining for HMGB1 in cervical intraepithelial neoplasia 1 (CIN\u20101), CIN\u20102, CIN\u20103, and invasive squamous cell carcinomas (ISCCs) were 69.4, 96.9, 100.0, and 100.0%, respectively. The differences between positive rates of patients with chronic cervicitis and various CINs as well as ISCCs were significant (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.005). The differences in positive staining rates between each two CIN groups, and differences between CIN\u20101\/2 and ISCCs, were also significant (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.005). The expression pattern of HMGB1 was generally correlated with that of p16 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). HMGB1 staining was observed in some p16\u2010negative specimens. HMGB1 immunostaining on a CB from gynecologic cytology specimens is potentially valuable for the screening of cervical lesions in cases with questionable cytology. Diagn. Cytopathol. 2014;42:802\u2013808. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.23091","type":"journal-article","created":{"date-parts":[[2014,2,18]],"date-time":"2014-02-18T13:41:33Z","timestamp":1392730893000},"page":"802-808","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic value of HMGB1 immunostaining on cell blocks from residual liquid\u2010based gynecologic cytology specimens"],"prefix":"10.1002","volume":"42","author":[{"given":"Li","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital, Sun Yat\u2010sen (Zhongshan) University Guangzhou 510080 People's Republic China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital, Sun Yat\u2010sen (Zhongshan) University Guangzhou 510080 People's Republic China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mian","family":"He","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics The First Affiliated Hospital Sun Yat\u2010sen (Zhongshan) University Guangzhou 510080 People's Republic China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital, Sun Yat\u2010sen (Zhongshan) University Guangzhou 510080 People's Republic China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guidong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital, Sun Yat\u2010sen (Zhongshan) University Guangzhou 510080 People's Republic China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huijuan","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital, Sun Yat\u2010sen (Zhongshan) University Guangzhou 510080 People's Republic China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shicong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital, Sun Yat\u2010sen (Zhongshan) University Guangzhou 510080 People's Republic China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liantang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital, Sun Yat\u2010sen (Zhongshan) University Guangzhou 510080 People's Republic China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shangwu","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biocontrol Guangdong Key Laboratory of Pharmaceutical Functional Genes Department of Biochemistry, School of Life Sciences, Sun Yat\u2010sen (Zhongshan) University Guangzhou 510275 People's Republic China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,2,18]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1594"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2009.11.014"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2010.01127.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvf198"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.94"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1622"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-009-0706-1"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2009.47"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2007.11.024"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990315)80:6<827::AID-IJC6>3.0.CO;2-P"},{"key":"e_1_2_5_12_1","first-page":"37","article-title":"The Papanicolaou test for cervical cancer detection. 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