{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:48:41Z","timestamp":1773661721697,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,2,28]],"date-time":"2014-02-28T00:00:00Z","timestamp":1393545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"CNPq INCT_if","award":["573663\/2008-4"],"award-info":[{"award-number":["573663\/2008-4"]}]},{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"crossref","award":["2010\/15919-1"],"award-info":[{"award-number":["2010\/15919-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:p>Studies about cervical carcinogenesis have demonstrated the increased expression of matrix\u2010metalloproteinase (MMP) according to the grade of cervical intraepithelial lesions. Considering the importance of innovative techniques to introduce noninvasive and rapid diagnoses for patients, this study aimed to perform MMP\u20109 immunocytochemistry in cervical smears according to the cytopathological diagnoses, in order to monitor MMP activity in cervical smears. This cross\u2010sectional study investigated the expression of MMP\u20109 in normal cervical smears, inflammatory cervical smears, squamous intraepithelial lesions, and cervical carcinoma. Cervical smears from 630 women were collected for cytopathological diagnoses and immunocytochemistry. Women with squamous intraepithelial lesions showed an increase in MMP\u20109 expression, with moderate to intense staining occurring with increasing cervical lesion grade. The prevalence of moderate to intense MMP\u20109 staining was 9% in normal cervical smears, 12% in cervical inflammation, 24% in low\u2010grade squamous intraepithelial lesion (LSIL), 92% in high\u2010grade squamous intraepithelial lesions (HSIL) and 100% in cervical carcinoma cases. In the specific case of LSIL, we found that association with MMP\u20109 is more evident when there is the simultaneous presence of an infectious agent. Thus, the expression of MMP\u20109 in cervical smears increases according to the grade of cervical lesion and LSIL in the presence of infectious agents showed higher MMP\u20109 expression than women with LSIL without infectious agents. Diagn. Cytopathol. 2014;42:827\u2013833. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.23124","type":"journal-article","created":{"date-parts":[[2014,2,28]],"date-time":"2014-02-28T05:30:41Z","timestamp":1393565441000},"page":"827-833","source":"Crossref","is-referenced-by-count":11,"title":["MMP\u20109 expression increases according to the grade of squamous intraepithelial lesion in cervical smears"],"prefix":"10.1002","volume":"42","author":[{"given":"Erika R.","family":"Matheus","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Pathology and Cytology, Department of Clinical Chemistry and Toxicology School of Pharmaceutical Sciences, University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco A.","family":"Zonta","sequence":"additional","affiliation":[{"name":"In Cito Laboratory\u2013Diagnostic Cytology S\u00e3o Paulo 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