{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:55:05Z","timestamp":1760298905998},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:p>Cytology is central in the diagnosis of malignancy in effusions. Ancillary techniques, mainly immunocytochemistry, have considerably improved the sensitivity but some 10% of all cases remain equivocal and require the addition of new diagnostic modalities. We have previously shown that strong nuclear telomerase activity determined with Telomere Repeat Amplification Protocol (TRAP) in situ is specific for malignant cells and could be a candidate for an additional test. Thirty effusions remaining diagnostically equivocal after the use of immunocytochemistry and the determination of the hyaluronan content were reviewed and their TRAP in situ reactivity was related to the definitive diagnoses based on all available data. There were seven effusions from patients with definitive benign diagnoses and 23 effusions from patients with definitive malignant diagnoses. Strong telomerase activity was seen only in effusions from patients with definitive malignant diagnosis, all effusions from patients with benign disease lacking strong telomerase activity, whereas eight of the malignant cases, including three cases of epithelial mesothelioma, showed strong reactivity. Strong nuclear TRAP in situ reactivity was demonstrated only in effusions from patients with verified malignant disease. Although the study is small, it suggests that TRAP in situ activity provides diagnostic information in about one\u2010third of effusions remaining cytologically equivocal after the use of current ancillary techniques. The most striking diagnostic improvement appears to be gained in epithelial mesotheliomas. Diagn. Cytopathol. 2014;42:1051\u20131057. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.23165","type":"journal-article","created":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T15:07:28Z","timestamp":1396364848000},"page":"1051-1057","source":"Crossref","is-referenced-by-count":7,"title":["Telomerase activity analyzed with TRAP in situ provides additional information in effusions remaining equivocal after immunocytochemistry and hyaluronan analysis"],"prefix":"10.1002","volume":"42","author":[{"given":"Elisabet","family":"Adell","sequence":"first","affiliation":[{"name":"Clinical Pathology University and Regional Laboratories, Region Sk\u00e5ne SUS Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annika","family":"Dejmek","sequence":"additional","affiliation":[{"name":"Clinical Pathology University and Regional Laboratories, Region Sk\u00e5ne SUS Malm\u00f6 Sweden"},{"name":"Department of Laboratory Medicine Lund University Malm\u00f6 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000325432"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2303.2000.00211.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20202"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1093\/clinchem\/40.5.777","article-title":"Clinical utility of liquid\u2010chromatographic analysis of effusions for hyaluronate content","volume":"40","author":"Nurminen M","year":"1994","journal-title":"Clin Chem"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2007.00468.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/pmj.79.936.569"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.2217\/fon.11.45"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2012.623"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.2043"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0339(200103)24:3<174::AID-DC1036>3.0.CO;2-8"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cllc.2012.01.007"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.03.00001303"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1093\/clinchem\/48.1.18","article-title":"Telomerase detection in body fluids","volume":"48","author":"Hess JL","year":"2002","journal-title":"Clin Chem"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.10352"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcts.2009.03.057"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000326092"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2007.02.040"},{"key":"e_1_2_6_19_1","first-page":"2470","article-title":"Telomerase assay for differentiating between malignancy\u2010related and nonmalignant ascites","volume":"5","author":"Tangkijvanich P","year":"1999","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_20_1","first-page":"199","article-title":"Cytological diagnosis and telomerase activity of cells in effusions of body cavities","volume":"6","author":"Toshima S","year":"1999","journal-title":"Oncol Rep"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.2.567"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/87.12.884"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(97)00062-2"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001622-199601000-00012"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00981880"},{"key":"e_1_2_6_26_1","first-page":"2100","article-title":"Cytological detection of telomerase activity using an in situ telomeric repeat amplification protocol assay","volume":"57","author":"Ohyashiki K","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0339(200101)24:1<11::AID-DC1001>3.0.CO;2-A"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800278"},{"key":"e_1_2_6_29_1","unstructured":"WHO.International classification of diseases ICD 10;2007. 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