{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T19:08:26Z","timestamp":1775070506979,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,8]],"date-time":"2015-12-08T00:00:00Z","timestamp":1449532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:p>Vaginal infections may affect susceptibility to and clearance of human papillomavirus (HPV) infection and chronic inflammation has been linked to carcinogenesis. This study aimed to evaluate the association between bacterial vaginosis (BV) and inflammatory response (IR) with the severity of cervical neoplasia in HPV\u2010infected women. HPV DNA was amplified using PGMY09\/11 primers and genotyping was performed using a reverse line blot hybridization assay in 211 cervical samples from women submitted to excision of the transformation zone. The bacterial flora was assessed in Papanicolaou stained smears, and positivity for BV was defined as \u226520% of clue cells. Present inflammatory response was defined as \u226530 neutrophils per field at 1000\u00d7 magnification. Age higher than 29 years (OR:1.91 95% CI 1.06\u20133.45), infections by the types 16 and\/or 18 (OR:1.92 95% CI 1.06\u20133.47), single or multiple infections associated with types 16 and\/or 18 (OR: 1.92 CI 95% 1.06\u20133.47), BV (OR: 3.54 95% CI 1.62\u20137.73) and IR (OR: 6.33 95% CI 3.06\u201313.07) were associated with severity of cervical neoplasia (CIN 2 or worse diagnoses), while not smoking showed a protective effect (OR: 0.51 95% CI 0.26\u20130.98). After controlling for confounding factors, BV(OR: 3.90 95% CI 1.64\u20139.29) and IR (OR: 6.43 95% CI 2.92\u201314.15) maintained their association with the severity of cervical neoplasia. Bacterial vaginosis and inflammatory response were independently associated with severity of cervical neoplasia in HPV\u2010positive women, which seems to suggest that the microenvironment would relate to the natural history of cervical neoplasia. Diagn. Cytopathol. 2016;44:80\u201386. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.23388","type":"journal-article","created":{"date-parts":[[2015,12,9]],"date-time":"2015-12-09T09:59:33Z","timestamp":1449655173000},"page":"80-86","source":"Crossref","is-referenced-by-count":34,"title":["Bacterial vaginosis and inflammatory response showed association with severity of cervical neoplasia in HPV\u2010positive women"],"prefix":"10.1002","volume":"44","author":[{"given":"Ju\u00e7ara Maria","family":"de Castro\u2010Sobrinho","sequence":"first","affiliation":[{"name":"School of Pharmacy Federal University of Minas Gerais Belo Horizonte MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia Helena","family":"Rabelo\u2010Santos","sequence":"additional","affiliation":[{"name":"School of Pharmacy Federal University of Goi\u00e1s Goi\u00e2nia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosane Ribeiro","family":"Fugueiredo\u2010Alves","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Federal University of Goi\u00e1s Goi\u00e2nia Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Derchain","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology State University of Campinas (UNICAMP) Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Ot\u00e1vio Z","family":"Sarian","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology State University of Campinas (UNICAMP) Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise R","family":"Pitta","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology State University of Campinas (UNICAMP) Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabete A","family":"Campos","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology State University of Campinas (UNICAMP) Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Carlos","family":"Zeferino","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology State University of Campinas (UNICAMP) Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/95.6.E2"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-9-16"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/319460"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"3751","DOI":"10.1128\/iai.58.11.3751-3758.1990","article-title":"Identification and partial characterization of a cytolytic toxin produced by Gardnerella vaginalis","volume":"58","author":"Rottini G","year":"1990","journal-title":"Infect Immun"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016349409006278"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.3802\/jgo.2009.20.1.39"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-11-10"},{"key":"e_1_2_5_9_1","first-page":"439","article-title":"Shift in flora suggestive of\/compatible with bacterial vaginosis: A public health review","volume":"108","author":"Morris M","year":"2001","journal-title":"BJOG"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/367658"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25637"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000079822"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20431"},{"key":"e_1_2_5_14_1","first-page":"1021","article-title":"An association of cervical inflammation with high\u2010grade cervical neoplasia in women infected with oncogenic human papillomavirus (HPV)","volume":"10","author":"Castle PE","year":"2001","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-200307000-00009"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2006.06.029"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910560311"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910430511"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1999.5358"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0b013e3181fde259"},{"key":"e_1_2_5_21_1","first-page":"36","volume-title":"World Health Organization. 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