{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T08:41:50Z","timestamp":1696668110532},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,1,23]],"date-time":"2016-01-23T00:00:00Z","timestamp":1453507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>HPV is known to have a predilection for infecting the transformation zone (TZ). Endocervical cells (EC) on a Pap test (PT) indicate that the cervical TZ has been sampled. Earlier repeat testing of women lacking EC is of little value in further detecting disease, thus a sample without EC is not necessarily inadequate. Both HPV testing and PT can be performed using a single sample; however, few studies have investigated the relationship between HPV results and TZ sampling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Specimens were collected following the ThinPrep<jats:sup>\u00ae<\/jats:sup> liquid\u2010based PT protocol. The Roche Cobas<jats:sup>\u00ae<\/jats:sup> HPV test was performed on post\u2010aliquot samples. Data was collected retrospectively on 500 patients: 250 consecutive cases of EC\u2212 and 250 of EC+ on PT. To maintain uniformity, we included only cases diagnosed as negative (NILM). We compared HPV test results within each category. As a positive control, five consecutive cases each of LSIL and HSIL were also reviewed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of NILM cases, 11 of 250 EC+ cases and 14 of 250 EC\u2212 cases were positive for hrHPV. HPV 16 was present in 5 of 11 EC\u2009+\u2009cases and in 1 of 14 EC\u2212 cases. Of LSIL cases, 1 of 5 EC+ cases was positive for hrHPV, and 2 of 5 EC\u2212 cases were positive for hrHPV. Of HSIL cases, 5 of 5 EC+ cases were hrHPV+. In the time period studied, only one case of EC\u2212 HSIL was found, which was positive for hrHPV.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Although our study did not prove a significant correlation between HPV testing results and EC on PT, more EC+ PTs were positive for HPV16 compared to EC\u2212 PTs. The absence of EC on PT does not appear to warrant re\u2010testing for HPV infection, though larger studies are required to determine the significance of low HPV 16 in PT without EC. Diagn. Cytopathol. 2016;44:280\u2013282. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.23436","type":"journal-article","created":{"date-parts":[[2016,1,23]],"date-time":"2016-01-23T09:02:50Z","timestamp":1453539770000},"page":"280-282","source":"Crossref","is-referenced-by-count":0,"title":["An analysis of human papillomavirus testing and endocervical component on pap tests: A pilot study using the Roche Cobas<sup>\u00ae<\/sup> assay"],"prefix":"10.1002","volume":"44","author":[{"given":"Kirsten J.","family":"Pierce","sequence":"first","affiliation":[{"name":"Dartmouth\u2010Hitchcock Medical Center Lebanon New Hampshire"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather S.","family":"Currens","sequence":"additional","affiliation":[{"name":"Dartmouth\u2010Hitchcock Medical Center Lebanon New Hampshire"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura J.","family":"Tafe","sequence":"additional","affiliation":[{"name":"Dartmouth\u2010Hitchcock Medical Center Lebanon New Hampshire"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory J.","family":"Tsongalis","sequence":"additional","affiliation":[{"name":"Dartmouth\u2010Hitchcock Medical Center Lebanon New Hampshire"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijayalakshmi","family":"Padmanabhan","sequence":"additional","affiliation":[{"name":"Dartmouth\u2010Hitchcock Medical Center Lebanon New Hampshire"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,23]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1016\/B978-1-4557-2865-7.00087-4","volume-title":"Abeloff's clinical oncology","author":"Jhingran A","year":"2014"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2050"},{"key":"e_1_2_5_4_1","unstructured":"CDC.Cervical Cancer Screening Guidelines for Average\u2010Risk Women [Internet].2015. 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