{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T14:05:31Z","timestamp":1785679531454,"version":"3.56.0"},"reference-count":29,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,4,28]],"date-time":"2016-04-28T00:00:00Z","timestamp":1461801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Loss of BAP1 by immunohistochemistry (IHC) and <jats:italic>CDKN2A<\/jats:italic>(p16) deletion by fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) have been proposed to distinguish malignant mesothelioma (MM) from atypical reactive mesothelial proliferations (ARMP) in effusions but it is uncertain whether both tests are needed routinely.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Paraffin embedded blocks from 67 effusions (32 MM, 35 ARMP) were evaluated with BAP1 IHC. 38 of them (17 MM, 21 ARMP) were also analyzed with <jats:italic>CDKN2A<\/jats:italic> (p16) FISH. Criteria for MM were absence of BAP1 nuclear staining in &gt;50% of atypical mesothelial cells in the presence of a positive internal control, and\/or <jats:italic>CDKN2A<\/jats:italic>(p16) homozygous or hemizygous deletion pattern in &gt;15% or &gt;41.5% of atypical mesothelial cells, respectively. Sensitivity (SS), specificity (SP), positive, and negative predictive values (PPV, NPV) for MM were calculated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>17 of 32 MM were correctly diagnosed by IHC (SS 53.1%, SP 85.7%, PPV 77.3%, NPV 64.3% for MM). In the subset of 38 cases studied by IHC and FISH, the SS, SP, PPV, and NPV of IHC for MM were 41.2, 81.0, 63.6, and 63.0%, respectively. 7 of 17 MM were diagnosed by FISH (SS 41.2%, SP 100%, PPV 100%, NPV 67.7% for MM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>BAP1 IHC and <jats:italic>CDKN2A<\/jats:italic>(p16) FISH yield similar sensitivities for MM in paired samples but FISH has a higher specificity. To diagnose effusions with atypical mesothelial cells, use of both tests is optimal. However, BAP1 IHC alone may be sufficient to diagnose MM when clinical, imaging, and cytological findings strongly suggest MM. Diagn. Cytopathol. 2016. \u00a9 2016 Wiley Periodicals, Inc. Diagn. Cytopathol. 2016;44:599\u2013606. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.23491","type":"journal-article","created":{"date-parts":[[2016,4,28]],"date-time":"2016-04-28T12:18:23Z","timestamp":1461845903000},"page":"599-606","source":"Crossref","is-referenced-by-count":65,"title":["BAP1 Immunostain and <i>CDKN2A<\/i> (p16) FISH Analysis"],"prefix":"10.1002","volume":"44","author":[{"given":"Ann E.","family":"Walts","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Cedars\u2010Sinai Medical Center Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenzo","family":"Hiroshima","sequence":"additional","affiliation":[{"name":"Department of Pathology Tokyo Women's Medical University Yachiyo Medical Center Yachiyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie M.","family":"McGregor","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Chicago Hospitals Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology Tokyo Women's Medical University Yachiyo Medical Center Yachiyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliya N.","family":"Husain","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Chicago Hospitals Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto M.","family":"Marchevsky","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Cedars\u2010Sinai Medical Center Los Angeles 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