{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T17:16:32Z","timestamp":1784740592315,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,5,24]],"date-time":"2016-05-24T00:00:00Z","timestamp":1464048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:p>Because most of malignant pleural mesothelioma (MPM) patients first present with pleural effusion, detection of mesothelioma cells on effusion smears is critical for early diagnosis. Recently, accumulating evidence indicating that the cytological diagnosis of MPM supported by ancillary techniques is as reliable as that based on histopathology has led to new guidelines for the cytopathologic diagnosis of MPM. Based on the guidelines, a combination of cytomorphological criteria and verification by ancillary techniques is required for the cytologic diagnosis of MPM. Detection of p16 homozygous deletion by fluorescence in situ hybridization (FISH) is the most reliable ancillary technique for differentiating MPM from reactive mesothelial cells (RMC) because of its relatively high sensitivity and extremely high specificity. We showed that the p16 deletion status of MPM cells in pleural effusions reflected that of the underlying invasive MPM tissues, indicating the usefulness of p16 FISH in effusion smear cytology for MPM diagnosis. Thus, for differentiating MPM from RMC, we propose to perform p16 FISH as often as possible. A positive p16 homozygous deletion supports the diagnosis of MPM. However, a negative result does not rule out the possibility of MPM. In such cases, a morphological assessment is critical. Therefore, we analyzed the morphological characteristics of p16 deletion\u2010positive mesothelioma cells using a combination of virtual microscopy and p16 FISH, and identified three morphological characteristics useful for the differentiation<jats:styled-content>,<\/jats:styled-content> including cell\u2010in\u2010cell engulfment with or without hump formation, multinucleate cells, and larger berry\u2010like cell aggregates. Diagn. Cytopathol. 2016;44:774\u2013780. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dc.23501","type":"journal-article","created":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T02:49:19Z","timestamp":1464144559000},"page":"774-780","source":"Crossref","is-referenced-by-count":27,"title":["Use of <i>p16<\/i> FISH for differential diagnosis of mesothelioma in smear preparations"],"prefix":"10.1002","volume":"44","author":[{"given":"Kazuki","family":"Nabeshima","sequence":"first","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and School of Medicine Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and School of Medicine Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Hamasaki","sequence":"additional","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and School of Medicine Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyuki","family":"Hida","sequence":"additional","affiliation":[{"name":"Department of Pathology Fukuoka University Hospital and School of Medicine Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiaki","family":"Kamei","sequence":"additional","affiliation":[{"name":"Department of Pathology PCL Japan Fukuoka, Pathology\u2010Cytology Center Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenzo","family":"Hiroshima","sequence":"additional","affiliation":[{"name":"Department of Pathology Tokyo Women's Medical University Yachiyo Medical Center Yachiyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Tsujimura","sequence":"additional","affiliation":[{"name":"Department of Pathology Hyogo College of Medicine Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunimitsu","family":"Kawahara","sequence":"additional","affiliation":[{"name":"Department of Pathology Osaka Prefectural Medical Center for Respiratory and Allergic Disease Habikino Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.19.8523"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2007.10.054"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.17.5604"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2007.081430"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.09-1951"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.5858\/133.8.1317"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2012-0214-OA"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000377697"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAT.0b013e32835bc848"},{"key":"e_1_2_11_11_1","first-page":"5547","article-title":"P16 alterations and deletion mapping of 9p21\u2010P22 in malignant mesothelioma","volume":"54","author":"Cheng JQ","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2006.04.011"},{"key":"e_1_2_11_13_1","first-page":"1227","article-title":"Neurofibromatosis type 2 (NF2) gene is somatically mutated in mesothelioma but not in lung cancer","volume":"55","author":"Sekido Y","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.855"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2012.02223.x"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-1008"},{"key":"e_1_2_11_17_1","first-page":"492","article-title":"Targeted\u2002next\u2010generation\u2002sequencing\u2002of\u2002cancer\u2002genes\u2002in\u2002advanced\u2002stage\u2002malignant\u2002pleural\u2002mesothelioma. 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