{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T23:38:42Z","timestamp":1773099522869,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,7,28]],"date-time":"2016-07-28T00:00:00Z","timestamp":1469664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>High\u2010risk Human papillomavirus (HR\u2010HPV)\u2010associated head and neck squamous cell carcinoma (HNSCC) is a distinct epidemiologic and pathologic disease. The data of HR\u2010HPV testing on fine\u2010needle aspiration (FNA) materials of cervical lymph nodes in patients with metastatic HNSCC are limited.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We retrospectively reviewed metastatic HNSCC cases with cervical lymph node FNAs which were tested for p16 immunohistochemistry (IHC) and HR\u2010HPV DNA in situ hybridization (ISH).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 87 cases studied, the overall adequacy rate of cell block material was 86% for HR\u2010HPV ISH, 93% for p16 IHC, 84% for both and 95% for either one. Pathologist\u2010performed FNAs showed significantly higher percentage of satisfactory specimens for p16 and HR\u2010HPV tests than clinician\u2010performed FNAs. Of the 73 cases adequate for both p16 IHC and HR\u2010HPV ISH, the concordant rate between p16 and HR\u2010HPV results was 78%. P16 positivity had sensitivity of 90% and specificity of 75% to predict primary oropharyngeal HNSCC, while HR\u2010HPV test showed a similar high sensitivity (91%) but a better specificity (94%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>FNAs of cervical lymph nodes yield adequate materials for reliable HR\u2010HPV testing in metastatic HNSCCs and should be incorporated into routine pathologic evaluation for these patients. The high concordance between p16 IHC and HR\u2010HPV ISH suggests p16 IHC may serve as a surrogate for HR\u2010HPV ISH in metastatic HNSCC cases with oropharyngeal origin and is helpful to pinpoint primary tumor site. Diagn. Cytopathol. 2016;44:792\u2013798. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.23548","type":"journal-article","created":{"date-parts":[[2016,7,28]],"date-time":"2016-07-28T05:16:53Z","timestamp":1469683013000},"page":"792-798","source":"Crossref","is-referenced-by-count":31,"title":["Fine\u2010needle aspiration of cervical lymph nodes yields adequate materials for accurate HPV testing in metastatic head and neck squamous cell carcinomas"],"prefix":"10.1002","volume":"44","author":[{"given":"Yanjun","family":"Hou","sequence":"first","affiliation":[{"name":"Department of Pathology Wexner Medical Center at the Ohio State University Columbus Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shweta","family":"Chaudhary","sequence":"additional","affiliation":[{"name":"Department of Pathology Wexner Medical Center at the Ohio State University Columbus Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rulong","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Pathology Wexner Medical Center at the Ohio State University Columbus Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zaibo","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology Wexner Medical Center at the Ohio State University Columbus Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,7,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.36.4596"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2014.05.004"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.14.1713"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(14)70471-1"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2013.54.5228"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70017-6"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2015.02.022"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPCZA4PSZCFHQ4"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-1690"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2013.04.009"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000375148"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2014.12.006"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000335730"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21375"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2015.61.6995"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318253a2d1"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23278"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21600"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2009.09.006"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2009.11.015"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318290427d"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20175"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21372"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdc.23548","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.23548","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T19:24:54Z","timestamp":1695497094000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.23548"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,28]]},"references-count":23,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1002\/dc.23548"],"URL":"https:\/\/doi.org\/10.1002\/dc.23548","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,28]]}}}