{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:15:43Z","timestamp":1790572543945,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T00:00:00Z","timestamp":1507593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009880","name":"Lazio Region","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009880","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The important diagnostic challenge facing the cytopathologist is whether a mesothelial proliferation on effusions represents a malignant mesothelioma (MM) or a benign mesothelial hyperplasia (MH). Here, we evaluated the diagnostic utility of BAP1 immunohistochemistry (IHC) in distinguishing between reactive and neoplastic mesothelial cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In pleural and peritoneal effusions from 147 patients with diagnosed MM or with a differential diagnosis of MM and MH, the expression of BAP1 was examined by IHC on paraffin\u2010embedded cell blocks (<jats:italic>n<\/jats:italic>\u2009=\u2009121) and biopsies (<jats:italic>n<\/jats:italic>\u2009=\u200944). Included were also synchronous and methacronous cytology\/biopsy pair samples. BAP1 IHC was evaluated for nuclear staining as positive or negative on target mesothelial cells, with appropriate internal control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In MM cases, loss of BAP1 nuclear staining was observed in 76.5% of the cell blocks and 47.5% of the biopsies. All BAP1\u2010negative cases with a differential diagnosis of benign and malignant mesothelial proliferations were MM at follow\u2010up. All MH cases, the 29% of epithelial MM and the 90% of nonepithelial MM, retained BAP1 expression. Synchronous and methacronous biopsy\/cytology pairs showed matching BAP1 results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In effusions with mesotheliomatous cells or atypical mesothelial cells of uncertain significance, negative BAP1 IHC strongly supports a diagnosis of MM. With prudence in interpreting immunostaining, BAP1 may be included in IHC panels for MM cytodiagnosis, given its high specificity and sensitivity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.23837","type":"journal-article","created":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T10:02:46Z","timestamp":1507629766000},"page":"9-14","source":"Crossref","is-referenced-by-count":43,"title":["Loss of <scp>BRCA1<\/scp>\u2010associated protein 1 (<scp>BAP<\/scp>1) expression is useful in diagnostic cytopathology of malignant mesothelioma in effusions"],"prefix":"10.1002","volume":"46","author":[{"given":"Ilaria","family":"Cozzi","sequence":"first","affiliation":[{"name":"Department of Radiological, Oncological and Anatomo\u2010Pathological Sciences Sapienza University Viale Regina, Rome 324\u201000161 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florina Anca","family":"Oprescu","sequence":"additional","affiliation":[{"name":"Department of Radiological, Oncological and Anatomo\u2010Pathological Sciences Sapienza University Viale Regina, Rome 324\u201000161 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Rullo","sequence":"additional","affiliation":[{"name":"Department of Radiological, Oncological and Anatomo\u2010Pathological Sciences Sapienza University Viale Regina, Rome 324\u201000161 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7530-4832","authenticated-orcid":false,"given":"Valeria","family":"Ascoli","sequence":"additional","affiliation":[{"name":"Department of Radiological, Oncological and Anatomo\u2010Pathological Sciences Sapienza University Viale Regina, Rome 324\u201000161 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12250"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2012-0214-OA"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2006.08.010"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2003.01686.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2012-0112-RA"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.12216"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP5KNL7QTELLYI"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2015.65"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2015.87"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-11-2375"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0000000000000471"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3520"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2012.02223.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000394"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2015.06.024"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2015.12.012"},{"key":"e_1_2_6_18_1","first-page":"1","article-title":"BAP1 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