{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T19:03:20Z","timestamp":1775934200758,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Several methods have been applied to detect death cells in tissue, cell culture, and fluid samples. We aimed to compare the cytological and immunocytochemical methods for detecting apoptotic cells in cervicovaginal smears.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cervicovaginal smears were taken from 102 women for various gynecological complaints. The slides were stained using the Papanicolaou (Pap)\u2010staining method for cytological evaluation. Cleaved caspase 3 (CC3) antibody was used to detect apoptosis by immunocytochemically, and H\u2010Score was used for the evaluation. In Pap\u2010stained smears, apoptosis was detected and evaluated using our new scoring system by the examination of morphological changes of epithelial cells such as apoptotic bodies, blebbing, karyopyknosis, karyorrhexis, and karyolysis. We used Kappa analysis to understand whether there is an agreement between cytological and immunocytochemical methods for detecting apoptotic cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cytological and immunocytochemical methods showed similarities at a moderate level (\u03ba = 0.482, <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001). The cytological and immunocytochemical scores were also similar to each other at a fair level (\u03ba = 0.373, <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001). Pap smears had a sensitivity of 64.40% (95% CI: 50.12\u201076.01), specificity of 86.04% (95% CI: 72.65\u201094.83), positive likelihood ratio of 4.62, negative likelihood ratio of 0.41, positive predictive value of 86.36%, negative predictive value of 63.79%, and overall probability of 73.53% compared to immunocytochemical staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results demonstrate that Pap smear and cytological scores alone were not good enough to identify apoptosis compared to the immunocytochemical studies. However, because of its high specificity, it may still be an adequate method to detect apoptotic cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.24316","type":"journal-article","created":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T04:38:33Z","timestamp":1569991113000},"page":"1277-1282","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of cytological and immunocytochemical methods for detecting apoptotic epithelial cells in cervicovaginal smears"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7413-4939","authenticated-orcid":false,"given":"Hanife Guler","family":"Donmez","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6362-787X","authenticated-orcid":false,"given":"Mehmet Sinan","family":"Beksac","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, Faculty of Medicine Hacettepe University  Ankara Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2017.133"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/nips.01519.2004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-007-0356-9"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2002-0090OC"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11307-014-0802-8"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540205000401"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/842037"},{"key":"e_1_2_6_9_1","first-page":"42","article-title":"High incidence of apoptosis in peripheral blood 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