{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:37:05Z","timestamp":1770824225744,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T00:00:00Z","timestamp":1583193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"JSPS KAKENHI","award":["JP16K10690"],"award-info":[{"award-number":["JP16K10690"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Liquid\u2010based cytology (LBC) allows immunohistochemistry (IHC), fluorescence in situ hybridization, and molecular testing to be performed in fixed cell materials. We examined the feasibility of subtyping and <jats:italic>EGFR<\/jats:italic> mutation testing of bronchoscopic samples from patients with lung cancer using cell blocks (CB) based on LBC fixation (LBC\u2010CB).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We included 35 consecutive patients with peripheral lung nodules who underwent endobronchial ultrasonography with a guide sheath in our hospital. Thirty of these patients were diagnosed with lung cancer by obtaining cytological samples. Cytological subtyping was performed with IHC using LBC\u2010CB, and the Cobas <jats:italic>EGFR<\/jats:italic> Mutation Test ver. 2 was performed using extracted genomic DNA from the LBC\u2010CB, formalin\u2010fixed paraffin\u2010embedded (FFPE) tissue, and matched plasma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 30 cases, 25 were classified cytomorphologically as adenocarcinoma (ADC, n = 17) and squamous\u2010cell carcinoma (SQCC, n = 8). The remaining five cases were classified by IHC as favor ADC (n = 3) and favor SQCC (n = 2) according to the WHO criteria. In the final ADC group (n = 20), <jats:italic>EGFR<\/jats:italic> mutations on the LBC\u2010CB were identified in eight cases (40%; 1 exon 19 deletion, 6 L858R, and 1 L861Q). Mutations in FFPE samples were identified in seven cases (35%) at the same site in each case. Plasma <jats:italic>EGFR<\/jats:italic> mutations were identified in four cases (20%) at the same site. The CB detection rate was higher than for FFPE and plasma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>LBC\u2010CB is suitable for subtyping and <jats:italic>EGFR<\/jats:italic> mutation testing in lung cancers.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.24397","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T14:18:09Z","timestamp":1583245089000},"page":"516-523","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Subtyping and <i>EGFR<\/i> mutation testing from blocks of cytological materials, based on liquid\u2010based cytology for lung cancer at bronchoscopic examinations"],"prefix":"10.1002","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0615-0072","authenticated-orcid":false,"given":"Ryota","family":"Tanaka","sequence":"first","affiliation":[{"name":"Department of Surgery Kyorin University School of Medicine  Tokyo 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