{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:08:52Z","timestamp":1787508532659,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T00:00:00Z","timestamp":1586476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81100697\/H1208"],"award-info":[{"award-number":["81100697\/H1208"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The limited sensitivity of Papanicolaou (Pap) cytology and the low specificity of HPV testing in detecting cervical or vaginal lesions means that either precancers are missed or women without lesions are overtreated. To improve performance outcomes, p16\/Ki\u201067 dual\u2010stain cytology has been introduced as a useful biomarker.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A prospective, cross\u2010sectional study was performed and included 599 patients. Clinical performance estimates of Pap cytology, HPV DNA assay, and p16\/Ki\u201067 dual\u2010stain cytology for the detection of CIN2+\/VAIN2+ were determined and compared.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The sensitivity and specificity of p16\/Ki\u201067 dual\u2010stain cytology in detecting histology proven CIN2+\/VAIN2+ was 91.6% and 95.0%, respectively, while that of Pap cytology was 42.1% and 95.2%, respectively, and that of HPV DNA testing was 100% and 41.6%, respectively. Among the three tests, the AUC of p16\/Ki\u201067 immunocytochemistry was the largest, both for detecting cervical lesions and vaginal lesions, at 0.932 and 0.966, respectively. Among women who were HPV 16\/18 positive or 12\u2010other hrHPV positive and Pap positive (\u2265ASCUS), dual staining reduced the number of unnecessary colposcopy referrals from 274 to 181. Among the women who were 12\u2010other hrHPV positive and Pap negative, dual staining could prevent underdiagnosis in six patients with CIN2+\/VAIN2+ when used as a triage marker. Dual staining also identified four women with high\u2010grade lesions detected by diagnostic conization but with negative colposcopy\u2010guided biopsy results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>p16\/Ki\u201067 dual staining may be a promising tool for predicting high\u2010grade cervical and vaginal lesions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dc.24427","type":"journal-article","created":{"date-parts":[[2020,4,10]],"date-time":"2020-04-10T11:50:04Z","timestamp":1586519404000},"page":"635-644","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Good performance of p16\/Ki\u201067 dual\u2010stain cytology for detection and post\u2010treatment surveillance of high\u2010grade\n                    <scp>CIN<\/scp>\n                    \/\n                    <scp>VAIN<\/scp>\n                    in a prospective, cross\u2010sectional study"],"prefix":"10.1002","volume":"48","author":[{"given":"Wei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology Affiliated Hospital of Qingdao University  Qingdao Shan Dong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinping","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Affiliated Hospital of Qingdao University  Qingdao Shan Dong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haicang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathology Affiliated Hospital of Qingdao University  Qingdao Shan Dong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Affiliated Hospital of Qingdao University  Qingdao Shan Dong 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