{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T08:10:36Z","timestamp":1777968636452,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T00:00:00Z","timestamp":1598572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Despite Aptima assay as the latest US Food Drug Administration (FDA)\u2010approved high\u2010risk human papillomavirus (hrHPV) test has been implemented as an adjunct in cervical cancer screening for years, histological follow\u2010up data remain limited with respect to its performance in women with ASC\u2010US Pap tests and positive hrHPV results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cases with results of ASC\u2010US cytology and positive hrHPV by Aptima assay during the period 06\/ 2015\u201302\/2017 were retrieved from archived pathology reports. Immediate histological follow\u2010up results were analyzed within 6 months interval after cotesting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among 4196 women with ASC\u2010US Pap tests and positive hrHPV, 51.1% of them had the immediate histological follow\u2010up within 6 months. With positive Aptima hrHPV as the adjunct, 46.5% (95%CI 46.2\u201346.8) of ASC\u2010US women were found to have cervical intraepithelial neoplasia type 1 (CIN1); 8.8% (95%CI 8.1\u20109.5) women were detected CIN2+ lesion including eight adenocarcinoma in\u2010situ (AIS)s. CIN2+ detection rates were highest in women under 25 (15.4%, n = 65), when comparison with different age cutoffs, younger age women had higher CIN2+ lesion detection rate than that in older group (P &lt;.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This is by far one of the largest retrospective studies to analyze the histological follow\u2010up results of ASC\u2010US women with positive hrHPV tested by Aptima hrHPV mRNA assay. The results indicated that younger women with ASC\u2010US and positive hrHPV testing have highest risk of developing high grade CIN lesions as compared to the older women. Lastly, with positive HPV as the adjunct, 55.3% (1186\/2145) of ASC\u2010US women will result in the positive finding on histological follow\u2010up.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.24592","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T21:56:03Z","timestamp":1598651763000},"page":"5-10","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Clinical performance of the aptima <scp>HPV<\/scp> assay in 4196 women with positive <scp>high\u2010risk HPV<\/scp> and <scp>ASC\u2010US<\/scp> cytology: A large women hospital experience"],"prefix":"10.1002","volume":"49","author":[{"given":"Tiannan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology, Magee\u2010Womens Hospital University of Pittsburgh Medical Center  Pennsylvania USA"},{"name":"Department of Pathology University of Southern California  Los Angeles California 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