{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T04:28:36Z","timestamp":1771302516084,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T00:00:00Z","timestamp":1605744000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Atypical glandular cells (AGC) as a diagnostic category in cervicovaginal cytology remains as a challenge to cytopathologists.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>The aim of the present study is to identify the cytological features helpful in categorizing AGC as reactive or neoplastic upon correlation with histology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The study was a retrospective review of cervical smears, with histopathological follow up, reported as glandular lesions for a period of 9 years. The architectural and nuclear features studied were adapted from The Bethesda System (TBS) to stratify the lesions as AGC, AGC\u2010FN (atypical glandular cells favour neoplasia) and adenocarcinoma. The cytological categories were correlated with histology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 89 cases of which 67 (AGC NOS = 34, AGC FN = 19, adenocarcinoma = 14) with histology were reviewed. Neoplastic lesions were encountered in 14 cases (34.6%). Of the cases diagnosed as AGC\u2010NOS, AGC\u2010FN and adenocarcinoma, 26.5%, 68.4% and 100% respectively were neoplastic on histopathology. Squamous lesions accounted for 14.9% of all the glandular lesions. Rosette or acinar formation and loss of polarity frequently observed in neoplastic lesions as compared to reactive changes (p = 0.0004, p = 0.001). Of the nuclear features, nuclear hyperchromasia or coarse clumping of chromatin along with nuclear membrane irregularity and nuclear pleomorphism was frequently associated with neoplastic lesions as compared to reactive conditions (p = 0.007, p = 0.001, p = 0.0002).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A diagnosis of AGC at cytology harbors significant number of malignant lesions when confirmed on biopsy. Architectural features complemented with nuclear characteristics helps in differentiating between reactive and neoplastic conditions. Hence stratifying glandular lesions at cytology according to TBS helps in the management.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.24668","type":"journal-article","created":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T05:55:03Z","timestamp":1605851703000},"page":"388-394","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Assessment of cytological features of glandular lesions of the cervix on conventional smear preparations\u2014a comprehensive study from a tertiary care hospital"],"prefix":"10.1002","volume":"49","author":[{"given":"Shweta","family":"Mantri","sequence":"first","affiliation":[{"name":"Department of Pathology St. John's Medical College  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gayatri","family":"Ravikumar","sequence":"additional","affiliation":[{"name":"Department of Pathology St. John's Medical College  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5767-9451","authenticated-orcid":false,"given":"Julian","family":"Crasta","sequence":"additional","affiliation":[{"name":"Department of Pathology St. John's Medical College  Bangalore India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-5851.96961"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/07357907.2014.880453"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.16.2114"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.10316"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1479-828X.2006.00678.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.16.2120"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2009.07.008"},{"issue":"1","key":"e_1_2_7_9_1","first-page":"117","article-title":"Comparative cytologic features of adenocarcinoma in situ of the uterine cervix","volume":"35","author":"Lee KR","year":"1990","journal-title":"Acta Cytol"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20782"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(199707)17:1<1::AID-DC1>3.0.CO;2-L"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20782"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000342744"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20799"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/101.6.708"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2019.06.006"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0104-42302011000200024"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2008.00568.x"},{"key":"e_1_2_7_19_1","first-page":"325","article-title":"The cytologic diagnosis of adenocarcinoma in situ of the cervix uteri and related lesions. 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