{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T03:24:01Z","timestamp":1771471441346,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,3,12]],"date-time":"2021-03-12T00:00:00Z","timestamp":1615507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pleomorphic adenoma (PA) is the most common benign salivary gland tumor. Fine\u2010needle aspiration (FNA) of PA exhibits variable combinations of bland ductal epithelial cells, myoepithelial cells, and characteristic magenta fibrillary stroma on Diff\u2013Quik\/Romanowsky stain. However, a cellular PA with scant chondromyxoid stroma can be a diagnostic challenge on FNA. Around 70% of PAs have a translocation involving <jats:italic>PLAG1<\/jats:italic> or <jats:italic>HMGA2<\/jats:italic>. The presence of either <jats:italic>PLAG1<\/jats:italic> or <jats:italic>HMGA2<\/jats:italic> fusion gene can be used to diagnose PA since they have not been reported in other salivary gland tumors except for carcinoma ex PA. In this case report, we describe a case of cellular PA initially diagnosed on FNA as a \u201clow grade salivary gland neoplasm, favor PA.\u201d RNA next\u2010generation sequencing performed on the cell block showed a <jats:italic>BOC\u2010PLAG1<\/jats:italic> fusion gene. The presence of <jats:italic>PLAG1<\/jats:italic> fusion gene in conjunction with cytomorphology supported a diagnosis of PA. The mass was surgically removed and proved to be a cellular PA with scattered foci of chondromyxoid and collagenous stroma. To our knowledge, this is the first reported PA bearing <jats:italic>BOC\u2010PLAG1<\/jats:italic>. RNA next\u2010generation sequencing performed on cytology specimens can be helpful in achieving a more specific diagnosis of salivary gland tumors.<\/jats:p>","DOI":"10.1002\/dc.24714","type":"journal-article","created":{"date-parts":[[2021,3,12]],"date-time":"2021-03-12T15:24:33Z","timestamp":1615562673000},"page":"790-792","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["<scp>BOC\u2010PLAG1<\/scp>, a new fusion gene of pleomorphic adenoma: Identified in a fine\u2010needle aspirate by <scp>RNA<\/scp> next\u2010generation sequencing"],"prefix":"10.1002","volume":"49","author":[{"given":"Jianming","family":"Pei","sequence":"first","affiliation":[{"name":"Department of Pathology Fox Chase Cancer Center  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey C.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology Fox Chase Cancer Center  Philadelphia Pennsylvania USA"},{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Lewis Katz School of Medicine at Temple University  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hormoz","family":"Ehya","sequence":"additional","affiliation":[{"name":"Department of Pathology Fox Chase Cancer Center  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0441-6973","authenticated-orcid":false,"given":"Shuanzeng","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pathology Fox Chase Cancer Center  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,3,12]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"185","volume-title":"WHO Classification of Head and Neck Tumours","author":"El\u2010Naggar 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