{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T16:04:59Z","timestamp":1783181099293,"version":"3.54.6"},"reference-count":16,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T00:00:00Z","timestamp":1619049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Secretory carcinoma (SC) of the salivary gland is a rare distinct clinicopathological entity characterized by the presence of the <jats:italic>ETV6\u2010NTRK3<\/jats:italic> fusion. Although the characteristic cytological features of SC have been recognized, its cytodiagnosis, especially differentiating SC from acinic cell carcinoma, is challenging. Recent studies have revealed that immunohistochemistry for pan\u2010tyrosine receptor kinase (Trk) and nuclear receptor subfamily 4 group member 3 (NR4A3) are specific for SC and acinic cell carcinoma, respectively. However, the usefulness of immunocytochemical detection of these markers in the diagnosis of SC has not been addressed. Hence, the present study aimed to analyze the usefulness of immunocytochemical staining for pan\u2010Trk and NR4A3 in the cytodiagnosis of SC. We enrolled eight patients with a histopathological diagnosis of SC who underwent preoperative fine\u2010needle aspiration cytological examination. The cytological characteristics were reviewed and immunocytochemical staining for pan\u2010Trk and NR4A3 was performed. The characteristic cytological features noted in the patient cohort included neoplastic cell clusters with a sheet\u2010like and papillary cluster arrangement as well as single cells. Additionally, neoplastic cells with mild to moderately enlarged nuclei and small nucleoli, multi\u2010vacuolated cytoplasm, and intracytoplasmic mucin were also observed. The immunocytochemical analyses clearly demonstrated that all eight specimens showed positive nuclear staining for pan\u2010Trk, but were negative for NR4A3 expression. Although all cases of SC do not always show positive immunoreactivity for pan\u2010Trk, immunocytochemical analysis for pan\u2010Trk may be useful for the cytodiagnosis of SC along with assessment of the characteristic cytological features.<\/jats:p>","DOI":"10.1002\/dc.24750","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T18:36:19Z","timestamp":1619116579000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Utility of an immunocytochemical analysis for <scp>pan\u2010Trk<\/scp> in the cytodiagnosis of secretory carcinoma of the salivary gland"],"prefix":"10.1002","volume":"49","author":[{"given":"Hiroko","family":"Ito","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Kansai Medical University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9585-4118","authenticated-orcid":false,"given":"Mitsuaki","family":"Ishida","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Kansai Medical University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Ebisu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Kansai Medical University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimiaki","family":"Okano","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Kansai Medical University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Sandoh","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Kansai Medical University  Osaka 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