{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:52:50Z","timestamp":1747147970018,"version":"3.40.5"},"reference-count":17,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The parasite Trichomonas vaginalis (<jats:italic>T. vaginalis<\/jats:italic>) causes one of the most common non\u2010viral sexually transmitted infections in humans. <jats:italic>T. vaginalis i<\/jats:italic>s notorious for its inconspicuous appearance in vaginal smears. It can be missed under the microscope.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>In the present study, we investigate the immunoreactivity of <jats:italic>T. vaginalis<\/jats:italic> to smooth muscle actin (SMA) in the vaginal smear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p><jats:italic>T. vaginalis<\/jats:italic> trophozoite and pseduocyst are immunoreactive for SMA in all of the study group cases (<jats:italic>n<\/jats:italic>\u00a0=\u00a021) and in none of the control group cases (<jats:italic>n<\/jats:italic>\u00a0=\u00a021). Thus, SMA immunostain is a sensitive method for the demonstration of <jats:italic>T. vaginalis<\/jats:italic>. Moreover, the protozoan attains a conspicuous and unique appearance. By SMA immunohistochemical stain, the apperance of <jats:italic>T. vaginalis<\/jats:italic> floated freely or located in the cytoplasm of the epithelial cells is easily identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We recommend performing SMA immunostain in every vaginal smear with clinical or pathologic suspicion of trichomoniasis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.24822","type":"journal-article","created":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T11:23:45Z","timestamp":1626953025000},"page":"1052-1055","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Smooth muscle actin immunostain is useful for the diagnosis of Trichomonas vaginalis in vaginal smears"],"prefix":"10.1002","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0998-2069","authenticated-orcid":false,"given":"Ni","family":"Fang","sequence":"first","affiliation":[{"name":"Department 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