{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T11:11:16Z","timestamp":1756897876472,"version":"3.40.5"},"reference-count":21,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,9,17]],"date-time":"2021-09-17T00:00:00Z","timestamp":1631836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cytological detection of chordoma cells in the serosal cavity is challenging because of its rare presentation. Herein, we report a case of chordoma showing malignant pleural effusion accompanied by pleuropulmonary metastases in a 68\u2010year\u2010old woman. Cytological analysis was performed using pleural fluid obtained following thoracentesis. Conventional cytological staining demonstrated few clusters of large, atypical cells characterized by epithelial cell\u2010like connectivity and rich cytoplasm with foamy and\/or multivacuolar changes. The nuclei of these atypical cells were large and either round or oval with no conspicuous irregularities in the nuclear membrane. Periodic acid\u2010Schiff staining of these atypical cells revealed fine granules in the cytoplasm. Giemsa staining showed foamy and\/or multivacuolar cytoplasm in these cells, with metachromatic mucoid stroma in the surroundings. Immunocytochemistry analysis using cellblock showed these cells to be positive for broad cytokeratins, epithelial membrane antigen, S100 protein, vimentin, and Brachyury. To the best of our knowledge, this is the first case report in which chordoma cells were cytologically detected in pleural effusions. Our findings also suggest that conventional cytology combined with cellblock immunocytochemistry can increase the accuracy of chordoma cell detection in the serosal cavity.<\/jats:p>","DOI":"10.1002\/dc.24832","type":"journal-article","created":{"date-parts":[[2021,9,17]],"date-time":"2021-09-17T08:52:15Z","timestamp":1631868735000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cytological detection of metastatic chordoma cells in pleural effusions: A case report"],"prefix":"10.1002","volume":"49","author":[{"given":"Mio","family":"Tamba\u2010Sakaguchi","sequence":"first","affiliation":[{"name":"Department of Diagnostic Pathology Jichi Medical University Hospital  Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5036-9282","authenticated-orcid":false,"given":"Hisashi","family":"Oshiro","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Jichi Medical University Hospital  Tochigi Japan"},{"name":"Department of Diagnostic Pathology Jichi Medical University Saitama Medical Center  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Mato","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Medicine, Department of Medicine Jichi Medical University  Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Kikkawa","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Jichi Medical University  Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teruaki","family":"Endo","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Jichi Medical University  Tochigi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miki","family":"Yanagita","sequence":"additional","affiliation":[{"name":"Department of Diagnostic 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