{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:52:53Z","timestamp":1747147973683,"version":"3.40.5"},"reference-count":9,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T00:00:00Z","timestamp":1628640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Syphilis is an infectious disease caused by <jats:italic>Treponema pallidum<\/jats:italic> (<jats:italic>T. pallidum<\/jats:italic>). A cervical smear is useful when screening for sexually transmitted diseases; however, <jats:italic>T. pallidum<\/jats:italic> is not detected in the usual Papanicolaou smear. We report the detection of <jats:italic>T. pallidum<\/jats:italic> by immunocytological examination of a cervical smear. A 22\u2010year\u2010old woman presented with nephrotic syndrome. On admission, we performed screening tests for infections, and her serology was positive for syphilis. A Papanicolaou cervical smear (Thin\u2010Prep) showed slight nuclear enlargement, nuclear irregularity, and mild hyperchromasia in the superficial cells, but no organism was detected. <jats:italic>T. pallidum<\/jats:italic> was detected in the remaining specimen using immunocytochemistry. We also detected the <jats:italic>T. pallidum<\/jats:italic> DNA in a cervical biopsy specimen by polymerase chain reaction (PCR). Our findings suggest that immunocytological examination and PCR assay examination are useful tests for syphilis diagnosis.<\/jats:p>","DOI":"10.1002\/dc.24849","type":"journal-article","created":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T12:17:45Z","timestamp":1628684265000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of <i>Treponema pallidum<\/i> by immunocytochemistry of cervical smear: A case report"],"prefix":"10.1002","volume":"49","author":[{"given":"Hiroshi","family":"Noguchi","sequence":"first","affiliation":[{"name":"Division of Pathology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takako","family":"Tokumitsu","sequence":"additional","affiliation":[{"name":"Division of Pathology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiteru","family":"Kuroki","sequence":"additional","affiliation":[{"name":"Division of Pathology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiko","family":"Minematsu","sequence":"additional","affiliation":[{"name":"Division of Pathology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujiro","family":"Asada","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sayaka","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Department of Nephrology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoko","family":"Ochiai","sequence":"additional","affiliation":[{"name":"Department of Nephrology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masao","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Department of Nephrology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shouichi","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Department of Nephrology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junji","family":"Onishi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyuki","family":"Kawagoe","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Sameshima","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Katsuragi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1171-2620","authenticated-orcid":false,"given":"Yuichiro","family":"Sato","sequence":"additional","affiliation":[{"name":"Division of Pathology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"},{"name":"Department of Diagnostic Pathology, University of Miyazaki Hospital, Faculty of Medicine University of Miyazaki  Miyazaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,8,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.73"},{"key":"e_1_2_8_3_1","unstructured":"Notification Trends Among Syphilis Cases in Japan. 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