{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T01:33:54Z","timestamp":1773020034468,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T00:00:00Z","timestamp":1635206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Preserving the optimal quality of DNA and RNA is mandatory for molecular testing in lung adenocarcinoma cytological smears (LACSs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>DNA and RNA were isolated from 90 frozen unstained and 46 May Gr\u00fcnwald Giemsa (MGG) stained LACSs prepared from bronchial washing (BW), bronchial brushing (BB), and pleural effusion (PE) samples during 3\u2009years. Concentrations of nucleic acids in all LACSs were assessed by spectrophotometric analysis. Fragmentation of DNA and RNA was determined by PCR amplification of selected genes. Amplicons of 100, 200, 300, 400, and 600\u2009bp were used for DNA and 108\u2009bp\u2010long <jats:italic>HPRT1<\/jats:italic> transcript fragment for RNA fragmentation analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among 90 frozen LACSs, significantly lower DNA concentrations of BB and RNA concentrations of BW samples frozen for 6\u201310\u2009months were observed in comparison with samples frozen for longer periods (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05). Among 46 paired LACSs, 44 (95.7%) frozen and 15 (32.6%) MGG\u2010stained samples showed 600\u2009bp\u2010long DNA amplicons. Statistically significant difference (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05) in the fragmentation of DNA between frozen and MGG\u2010stained LACSs was observed (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05), with DNA being less fragmented in frozen LACSs. In addition, 33 (71.7%) frozen and 36 (78.2%) MGG\u2010stained LASCs showed <jats:italic>HPRT1<\/jats:italic> gene amplicon of 108\u2009bp. RNA was less fragmented in 3\u2010year old MGG\u2010stained samples than in LACSs frozen for 3\u2009years.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>DNA and RNA extracted from frozen and MGG\u2010stained LACSs showed different results depending on the time of storage and\/or type of samples, but in general all samples had adequate quantity and quality for downstream molecular testing.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.24896","type":"journal-article","created":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T12:26:43Z","timestamp":1635251203000},"page":"1244-1250","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Influence of <scp>deep\u2010freezing<\/scp> and <scp>MGG<\/scp> staining on <scp>DNA<\/scp> and <scp>RNA<\/scp> quality in different types of lung adenocarcinoma cytological smears"],"prefix":"10.1002","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6791-5289","authenticated-orcid":false,"given":"Suzana","family":"Harabajsa","sequence":"first","affiliation":[{"name":"Department of Pathology and Cytology, Division of Pulmonary Cytology Jordanovac University Hospital Centre Zagreb  Zagreb Croatia"},{"name":"Department for Biology, Division of Molecular Biology, Faculty of Science University of Zagreb  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerija","family":"Begi\u0107","sequence":"additional","affiliation":[{"name":"Department for Biology, Division of Molecular Biology, Faculty of Science University of Zagreb  Zagreb Croatia"},{"name":"Primary School \u201cSesvetski Kraljevec\u201d  Sesvetski Kraljevec Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Gr\u0161kovi\u0107","sequence":"additional","affiliation":[{"name":"Department for Biology, Division of Molecular Biology, Faculty of Science University of Zagreb  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vesna","family":"\u0160imi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cytology, Division of Pulmonary Cytology Jordanovac University Hospital Centre Zagreb  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo\u017eica Vrabec","family":"Branica","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cytology, Division of Pulmonary Cytology Jordanovac University Hospital Centre Zagreb  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonja","family":"Badovinac","sequence":"additional","affiliation":[{"name":"Department of Respiratory Diseases Jordanovac University Hospital Centre Zagreb  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"Smojver\u2010Je\u017eek","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cytology, Division of Pulmonary Cytology Jordanovac University Hospital Centre Zagreb  Zagreb Croatia"},{"name":"School of Medicine University of Zagreb  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Kora\u0107","sequence":"additional","affiliation":[{"name":"Department for Biology, Division of Molecular Biology, Faculty of Science University of Zagreb  Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e318206a221"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13000-016-0544-9"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.14694\/EdBook_AM.2012.32.44"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12325"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22318"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21276"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016-0091-SA"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21762"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21850"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2017-204561"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2014-202404"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdv613"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14714"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2011.00887.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000326380"},{"issue":"10","key":"e_1_2_9_17_1","first-page":"1672","article-title":"Are unstained smears, effective tools in the evaluation of the cytological sample adequacy?","volume":"6","author":"Hemalatha AL","year":"2012","journal-title":"J Clin Diagn Res"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21467"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21771"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-018-0138-z"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.24896","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dc.24896","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.24896","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T05:20:20Z","timestamp":1693200020000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.24896"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,26]]},"references-count":20,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/dc.24896"],"URL":"https:\/\/doi.org\/10.1002\/dc.24896","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,26]]},"assertion":[{"value":"2021-07-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}