{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T07:47:49Z","timestamp":1792223269381,"version":"4.4.0"},"reference-count":18,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T00:00:00Z","timestamp":1635206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Thyroid nodules with indeterminate cytology represent up to 30% of cases. Molecular testing is now highly recommended to improve management. This study aimed to evaluate the use of the Idylla\u2122 <jats:italic>NRAS<\/jats:italic>\/<jats:italic>BRAF<\/jats:italic> mutation test, a rapid and automated polymerase chain reaction (PCR) assay validated for fixed paraffin\u2010embedded use, on residual thyroid liquid\u2010based fine\u2010needle aspiration (LB\u2010FNA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Concordance between mutations detected by the Idylla\u2122 assay and the gold\u2010standard qPCR was assessed by splitting in two aliquots 31 <jats:italic>BRAF<\/jats:italic> or <jats:italic>RAS<\/jats:italic> mutated and 5 non\u2010mutated LB\u2010FNA samples. Samples were obtained either from simulated FNA after thyroidectomy or from FNA obtained during routine care. A third aliquot was used to assess the limit of detection of Idylla\u2122 for five mutated samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The Idylla\u2122 assay showed a sensitivity of 97% and a specificity of 83% as results were concordant for 34 out of 36 samples. One discordant sample concerned a BRAF p.K601E\u2010mutation which is not detected by the Idylla\u2122 cartridge. The other showed a false\u2010positive NRAS p.A146T detection and a weak BRAF p.V600E detection. The limit of detection of the Idylla\u2122 assay was not reached by the dilution assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Idylla\u2122 <jats:italic>NRAS<\/jats:italic>\/<jats:italic>BRAF<\/jats:italic> mutation testing can be performed on residual thyroid LB\u2010FNA, using low DNA quantity input, thus not requiring a dedicated sample. The Idylla\u2122 <jats:italic>NRAS<\/jats:italic>\/<jats:italic>BRAF<\/jats:italic> assay offers a quick and easy first step for analyzing the main molecular alterations in indeterminate thyroid nodules, hence improving diagnostic management.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.24897","type":"journal-article","created":{"date-parts":[[2021,10,26]],"date-time":"2021-10-26T09:24:43Z","timestamp":1635240283000},"page":"1265-1271","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Feasibility and performance of the Idylla\u2122 <scp><i>NRAS<\/i><\/scp>\/<scp><i>BRAF<\/i><\/scp> cartridge mutation assay on thyroid liquid\u2010based fine\u2010needle aspiration"],"prefix":"10.1002","volume":"49","author":[{"given":"Maud","family":"Hamadou","sequence":"first","affiliation":[{"name":"D\u00e9partement de Biochimie et de Biologie Mol\u00e9culaire Centre Hospitalier Lyon Sud, Hospices Civils de Lyon  Pierre B\u00e9nite France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Lopez","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biochimie et de Biologie Mol\u00e9culaire Centre Hospitalier Lyon Sud, Hospices Civils de Lyon  Pierre B\u00e9nite France"},{"name":"Cancer Research Center of Lyon, Team EMT and Cancer Cell Plasticity, Lyon 1 University  Lyon France"},{"name":"Facult\u00e9 de M\u00e9decine et de Ma\u00efeutique Lyon Sud, Facult\u00e9 de M\u00e9decine Lyon Est Universit\u00e9 Lyon 1  Villeurbanne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazim","family":"Benzerdjeb","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine et de Ma\u00efeutique Lyon Sud, Facult\u00e9 de M\u00e9decine Lyon Est Universit\u00e9 Lyon 1  Villeurbanne France"},{"name":"D\u00e9partement d'Anatomie et Cytologie Pathologiques Centre Hospitalier Lyon Sud, Hospices Civils de Lyon  Pierre B\u00e9nite 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