{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T01:40:51Z","timestamp":1778895651437,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,12,29]],"date-time":"2021-12-29T00:00:00Z","timestamp":1640736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>COVID 19 has been rapidly spreading across the globe. As a result of alteration of the immune milieu by COVID 19 and its treatment, there has been a rise in opportunistic fungal infections particularly Mucormycosis in these patients. Delay in diagnosis of these fungal infections can be fatal. The usual diagnostic modalities used to detect Mucor include potassium hydroxide (KOH) mount, fungal culture, and histopathology. Since histopathology and fungal culture have a long turnaround time we are dependent on KOH mount for rapid results. Here we investigate the role of stained cytology smears in the rapid diagnosis of Mucormycosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A prospective observational study was conducted in a tertiary health care hospital on samples of patients clinically suspected to have Mucormycosis. We performed May Grunwald Giemsa (MGG) and Papanicolaou (PAP) stains on the remnant samples of nasal swabs\/scrapings\/biopsies after KOH test and fungal culture. We took 16 KOH positive and 16 KOH negative samples. We also examined 16 fresh samples from patients whose earlier samples were reported to be negative on KOH test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The 6\/16 KOH positive samples were found to be positive on stained cytology smears and 2 were mixed infections wherein both Mucor and Aspergillus were seen. The 4\/16 KOH negative samples were positive for Mucor with one sample having both Mucor and Aspergillus. The 3\/16 repeat samples which were earlier negative on KOH test were positive for Mucor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Stained cytology smears if used in conjunction with KOH test can increase the overall sensitivity of detection of Mucormycosis and mixed infections.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.24924","type":"journal-article","created":{"date-parts":[[2021,12,30]],"date-time":"2021-12-30T07:46:47Z","timestamp":1640850407000},"page":"93-98","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Utility of <scp>MGG<\/scp> and Papanicolaou stained smears in the detection of Mucormycosis in nasal swab\/scraping\/biopsy samples of <scp>COVID<\/scp> 19 patients"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1077-2576","authenticated-orcid":false,"given":"Ashish C.","family":"Philip","sequence":"first","affiliation":[{"name":"Department of Pathology University College of Medical Sciences, University of Delhi  Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prarthna","family":"Madan","sequence":"additional","affiliation":[{"name":"Department of Pathology University College of Medical Sciences, University of Delhi  Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonal","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Pathology University College of Medical Sciences, University of Delhi  Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shukla","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Microbiology University College of Medical Sciences, University of Delhi  Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12,29]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"COVID Live Update.200 264 356 cases and 4 258 693 deaths from the coronavirus: Worldometer Worldometersinfo;2021.https:\/\/www.worldometers.info\/coronavirus\/"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-020-00472-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofab016"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(21)00265-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.5858\/2001-125-0375-HFOZ"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13293"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.18203\/issn.2455-4529.IntJResDermatol20192107"},{"issue":"5","key":"e_1_2_8_9_1","first-page":"420","article-title":"A novel contrast stain for the rapid diagnosis of Dermatophytoses: a cross\u2010sectional comparative study of Chicago sky blue 6B stain, potassium hydroxide mount and culture","volume":"64","author":"Baddireddy K","year":"2019","journal-title":"Indian J Dermatol"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.2.341"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02803"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-013-0434-3"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7040298"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.15190\/d.2021.5"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12070-019-01774-z"},{"issue":"21","key":"e_1_2_8_16_1","first-page":"288","article-title":"The surge in Covid related mucormycosis","volume":"0163","author":"Chandra S","year":"2021","journal-title":"J Infect"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(20)30079-5"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974-2700.42203"},{"issue":"2","key":"e_1_2_8_19_1","first-page":"24","article-title":"Symptoms and treatment strategy of black fungus in Covid\u201019 patients","volume":"5","author":"Kameshwaran S","year":"2021","journal-title":"Int J Pharmacol Clin Res"},{"key":"e_1_2_8_20_1","first-page":"1","article-title":"Invasive fungal disease complicating COVID\u201019: when it rains, it spores","volume":"92103","author":"Hoenigl M","year":"2020","journal-title":"Clin Infect Dis"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1470-9465.2004.00843.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.4103\/2319-7250.296851"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof6040265"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00053-10"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.2.236"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.152900"},{"key":"e_1_2_8_27_1","unstructured":"Mucormycosis in COVID\u201019 | AIIMS Covid Information Portal. 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