{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T09:25:56Z","timestamp":1748251556357,"version":"3.40.5"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T00:00:00Z","timestamp":1643587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nasopharyngeal carcinoma (NPC) is an Epstein\u2013Barr virus (EBV)\u2010associated tumor with a high incidence in Asian countries. NPC is a type of squamous cell carcinoma originating from the nasopharyngeal mucosa. Although rare, NPCs show some uncommon histologic variants; these variations remain to be understood. We described the cytologic characteristics of a rare NPC variant with abundant intracytoplasmic mucin. A 37\u2010year\u2010old Japanese man presented to our hospital with bilateral ear discomfort and palpable lymph nodes. Nasopharyngeal biopsy showed tumor cells with abundant intracytoplasmic, Alcian blue\u2010PAS\u2010positive mucin. Immunohistochemical analysis demonstrated that the tumor cells were positive for p40 and p53. Epstein\u2013Barr encoding region (EBER) <jats:italic>in situ<\/jats:italic> hybridization (ISH) showed EBV infection of the tumor cells. Fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) using MAML2 break\u2010apart probes did not show split signals. Fine needle aspiration biopsy (FNAB) cytology of the metastatic lymph nodes was also performed. Smear samples had a necrotic and inflammatory background with both lymphocyte and neutrophil infiltration. Highly cellular tumor clusters and dispersed cells with naked nuclei were observed. The tumor cells showed a clear cytoplasm with distinct cell borders. Intracytoplasmic mucin pushing the nucleus to the periphery was observed in the scattered tumor cells in a liquid\u2010based cytology sample. Given these findings, the final diagnosis was advanced nasopharyngeal carcinoma; cisplatin\u2010based chemoradiation therapy was performed as the first\u2010line treatment. The tumor recurred 8\u2009months after completing the treatment. The recurrent nasopharyngeal tumor was a typical non\u2010keratinizing NPC and lacked intracytoplasmic mucin.<\/jats:p>","DOI":"10.1002\/dc.24934","type":"journal-article","created":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T18:23:32Z","timestamp":1643653412000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A rare case of intracytoplasmic mucin\u2010rich nasopharyngeal carcinoma"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1060-9018","authenticated-orcid":false,"given":"Yohei","family":"Yamaguchi","sequence":"first","affiliation":[{"name":"Department of Pathology University of Yamanashi  Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6273-2192","authenticated-orcid":false,"given":"Toru","family":"Odate","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Yamanashi  Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumiko","family":"Nakazawa","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Yamanashi  Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Oishi","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Yamanashi  Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9577-0320","authenticated-orcid":false,"given":"Kunio","family":"Mochizuki","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Yamanashi  Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Radiology University of Yamanashi  Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiwako","family":"Horiuchi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery, Interdisciplinary Graduate School of Medicine University of Yamanashi  Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery, Interdisciplinary Graduate School of Medicine University of Yamanashi  Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiju","family":"Sakurai","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery, Interdisciplinary Graduate School of Medicine University of Yamanashi  Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Yamanashi  Yamanashi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,31]]},"reference":[{"volume-title":"WHO Classification of Head and Neck Tumours","year":"2017","author":"EI\u2010NaggarAK CJ","key":"e_1_2_7_2_1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2015.02.021"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-018-0991-6"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200101000-00009"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.5858\/2000-124-1369-EBVRDA"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20216"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.10218"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(199809)19:3<168::AID-DC2>3.0.CO;2-9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.10197"},{"issue":"4","key":"e_1_2_7_11_1","first-page":"410","article-title":"Paucilymphoid non\u2010keratinizing nasopharyngeal carcinoma with prominent stromal desmoplasia: an unusual case reported with brief comments on uncommon histological variants","volume":"4","author":"Petersson F","year":"2011","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-006-0277-3"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300060514543041"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/829349"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21761"},{"volume-title":"Diagnostic Pathology. Cytopathology","year":"2018","author":"Dina RM","key":"e_1_2_7_16_1"},{"key":"e_1_2_7_17_1","unstructured":"RajaR SeethalaMIW BullockMJ et al.Protocol for the examination of specimens from patients with cancers of the pharynx;2021.https:\/\/documents.cap.org\/protocols\/HN.Pharynx_4.1.1.0.REL_CAPCP.pdf"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.24934","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dc.24934","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.24934","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T17:17:00Z","timestamp":1692983820000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.24934"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,31]]},"references-count":16,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["10.1002\/dc.24934"],"URL":"https:\/\/doi.org\/10.1002\/dc.24934","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"type":"print","value":"8755-1039"},{"type":"electronic","value":"1097-0339"}],"subject":[],"published":{"date-parts":[[2022,1,31]]},"assertion":[{"value":"2021-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}