{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T15:27:46Z","timestamp":1784820466448,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The majority of germ cell tumors (GCTs) are characterized by iso\u2010chromosome 12p (i12p) abnormality. The aim of this study is to review the cytomorphologic features and analyze the utility of i12p fluorescent in\u2010situ hybridization (FISH) test in diagnosing metastatic GCTs primarily evaluated by cytologic techniques in patients without prior history of GCTs. The laboratory information system was queried over a period of 10\u2009years to search for cases where i12p FISH test was requested on cytology material. FISH test was performed using TelVysion 12p telomeric probe and CEP 12 centromere probe on cell\u2010blocks. A ratio of 12ptel\/CEP12 signal of 1.4 or greater was considered as positive. Patient demographics, clinical presentation, cytopathologic findings, and follow\u2010up surgical resection data were reviewed and correlated. A total of three cases were identified, all men (age range 31\u201360\u2009years). Cytologic diagnoses were favor metastatic embryonal carcinoma (Case 1, retroperitoneal fluid FNA), metastatic yolk sac tumor\/YST (Case 2, lung mass FNA) and adenocarcinoma, likely representing a somatic\u2010type malignancy (SM) arising from a preexisting GCT (Case 3, retroperitoneal mass FNA). This limited study demonstrated high sensitivity for detecting i12p abnormality by FISH test performed on cell blocks. The cytomorphology of extra\u2010gonadal GCTs varies according to the histologic subtype. Sarcomatoid morphology of YST, SM or mixed GCTs further complicates cytology evaluation. FISH test for detection of i12p performed on cell\u2010blocks is extremely useful in establishing germ cell origin of these metastatic GTCs with unusual cytomorphology and guides management in patients without prior history of GCTs.<\/jats:p>","DOI":"10.1002\/dc.24980","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T13:33:27Z","timestamp":1653485607000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Fluorescent <scp>in\u2010situ<\/scp> hybridization test performed on cell blocks is useful for establishing germ cell origin of metastatic tumors with unusual clinical presentation"],"prefix":"10.1002","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1960-9847","authenticated-orcid":false,"given":"Swati P.","family":"Satturwar","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Pittsburgh Medical Center  Pittsburgh Pennsylvania USA"},{"name":"Department of Pathology, Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5670-6254","authenticated-orcid":false,"given":"Samer N.","family":"Khader","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Pittsburgh Medical Center  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0316-1267","authenticated-orcid":false,"given":"Gabriela M.","family":"Quiroga\u2010Garza","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Pittsburgh Medical Center  Pittsburgh Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102605.2011.01245.x"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0046\u20108177(83)80004\u20105"},{"key":"e_1_2_13_4_1","unstructured":"UlbrightTM AminMB BalzerBet alGerm cell tumors. 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