{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T05:39:40Z","timestamp":1775194780376,"version":"3.50.1"},"reference-count":8,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cornflake artifacts are artifacts that commonly occur while the mounted medium starts to evaporate before coverslipping. This study aimed to determine factors contributing to the occurrence of these artifacts in Papanicolaou (Pap) smears. Residual specimens were used after cytology to microscopically evaluate various effects on cornflake artifacts. Four SurePath\u2122 liquid cytology (LBC) cell specimens, diagnosed as negative for intraepithelial lesions or malignancy (NILM), were used. Each LBC smear was subjected to Pap staining under four different conditions (A, without air\u2010drying; B, air\u2010drying after dehydration; C, air\u2010drying after xylene immersion; and D, air\u2010drying after dehydration and xylene immersion) using two methods: conventional and poor dehydration. Cornflake artifacts were not observed in A and B in Pap staining. By contrast, cornflake artifacts were observed in conventional and poor dehydration methods when dried after xylene immersion. When comparing the four conditions, smears B and D, which were both air\u2010dried after dehydration, had fewer cornflake artifacts than smear C, which was air\u2010dried only after xylene. Therefore, the remaining water in the cells due to poor dehydration during xylene immersion is found to result in the development of cornflake artifacts. The present study revealed that cornflake artifacts in Pap smears are caused by poor dehydration in addition to drying before mounting.<\/jats:p>","DOI":"10.1002\/dc.25002","type":"journal-article","created":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T07:14:46Z","timestamp":1655450086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Causation of cornflake artifacts: Possible association of poor dehydration with drying before mounting in <scp>Papanicolaou<\/scp> stain"],"prefix":"10.1002","volume":"50","author":[{"given":"Kaori","family":"Okayama","sequence":"first","affiliation":[{"name":"Department of Medical Technology, Faculty of Health Sciences Gunma Paz University  Takasaki\u2010shi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyoshi","family":"Ishii","sequence":"additional","affiliation":[{"name":"Genki Plaza Medical Center for Health Care  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiko","family":"Fujii","sequence":"additional","affiliation":[{"name":"Genki Plaza Medical Center for Health Care  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mizue","family":"Oda","sequence":"additional","affiliation":[{"name":"Genki Plaza Medical Center for Health Care  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6152-3217","authenticated-orcid":false,"given":"Mitsuaki","family":"Okodo","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Faculty of Health Sciences Kyorin University  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,17]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1603","volume-title":"Koss' Diagnostic Cytology and its Histopathologic Bases","author":"Koss LG","year":"2006"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-276-2_18"},{"key":"e_1_2_9_4_1","first-page":"124","volume-title":"The Art and Science of Cytopathology: Exfoliative Cytology","author":"DeMay RM","year":"2012"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(199808)19:2<138::AID-DC15>3.0.CO;2-G"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000509604"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.5005\/jp\/books\/14221"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23965"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2014.11.076"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.25002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dc.25002","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.25002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T08:13:47Z","timestamp":1692778427000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.25002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,17]]},"references-count":8,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1002\/dc.25002"],"URL":"https:\/\/doi.org\/10.1002\/dc.25002","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,17]]},"assertion":[{"value":"2022-04-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}