{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T06:26:06Z","timestamp":1761719166378,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Pancreatic ductal adenocarcinoma (PDAC) represents the most common primary pancreatic malignancy. An understanding of the cytomorphologic features of conventional ductal adenocarcinoma and its variants is important to ensure accurate diagnoses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The clinicopathological and cytological data of serous fluids in PDAC patients were obtained from the electronic medical records and pathology database. All samples were analyzed and reclassified according to the \u201cThe International System for Reporting Serous Fluid Cytopathology\u201d guidelines. Cytomorphologic features were examined with SurePath automatically prepared slides and stained using the Pap method in malignant (MAL) effusion specimens from 21 patients with PDAC. Immunocytochemical staining was conducted on 12 cell blocks from MAL PDAC effusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 137 serous fluids specimens of PDACs were included, among which 61 (44.5%), 9 (6.6%), 13 (9.5%), 52 (38.0%), and 2 (1.5%) patients were classified into malignancy, suspicious for malignancy, atypia of undetermined significance, negative for malignancy and nondiagnostic groups, respectively. The key cytologic features for the conventional type of PDAC included cohesive clusters of ductal cells in glandular crowding and disorganized \u201cdrunken honeycomb\u201d pattern or intercalated duct\u2010like structure with anisonucleosis, cytoplasmic vacuoles, and concomitant \u201cIndian\u2010file\u201d configuration. Undifferentiated carcinoma was comprised of enlarged, undifferentiated, pleomorphic MAL cells. Adenosquamous carcinoma could show glandular and\/or squamous differentiation. Colloid carcinoma was composed of three\u2010dimensional cancer cell clusters floating in thick mucin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Crowding and disorganized \u201cdrunken honeycomb\u201d pattern or intercalated duct\u2010like structure with anisonucleosis, may represent an important clue for diagnosing PDAC in serous fluids. Immunocytochemical staining in combination with review of medical records and cytomorphological data can serve as useful adjuncts for distinguishing between PDAC and its variants.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.25014","type":"journal-article","created":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T11:09:24Z","timestamp":1657883364000},"page":"471-481","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cytomorphological and immunohistochemical features of pancreatic ductal adenocarcinoma in serous fluids"],"prefix":"10.1002","volume":"50","author":[{"given":"Xiao\u2010Yu","family":"Nie","sequence":"first","affiliation":[{"name":"Department of Pathology, Yueyang Integrated Traditional Chinese and Western Medicine Hospital Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yueyang Integrated Traditional Chinese and Western Medicine Hospital Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shou\u2010Mei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yueyang Integrated Traditional Chinese and Western Medicine Hospital Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yueyang Integrated Traditional Chinese and Western Medicine Hospital Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun\u2010Cui","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yueyang Integrated Traditional Chinese and Western Medicine Hospital Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yueyang Integrated Traditional Chinese and Western Medicine Hospital Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai\u2010Yan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yueyang Integrated Traditional Chinese and Western Medicine Hospital Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3066-2764","authenticated-orcid":false,"given":"Shu\u2010Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yueyang Integrated Traditional Chinese and Western Medicine Hospital Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,15]]},"reference":[{"volume-title":"Who Classification of Tumours Editorial Board. Who Classification of Tumours\u2010Digestive System Tumours","year":"2019","author":"Gill AJ","key":"e_1_2_9_2_1"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.13113"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24804"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-53908-5"},{"issue":"3","key":"e_1_2_9_6_1","first-page":"311","article-title":"Cytopathology of exocrine pancreatic carcinoma in effusions","volume":"35","author":"Di Bonito L","year":"1991","journal-title":"Acta Cytol"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24720"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24440"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22345"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24653"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000507311"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasc.2020.05.015"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24864"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.13089"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24663"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-22433-2"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-16589-9"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24901"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22514"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqaa058"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21202"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.25014","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dc.25014","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.25014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T08:13:32Z","timestamp":1692778412000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.25014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,15]]},"references-count":20,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1002\/dc.25014"],"URL":"https:\/\/doi.org\/10.1002\/dc.25014","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"type":"print","value":"8755-1039"},{"type":"electronic","value":"1097-0339"}],"subject":[],"published":{"date-parts":[[2022,7,15]]},"assertion":[{"value":"2022-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}