{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:50:32Z","timestamp":1790707832197,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T00:00:00Z","timestamp":1659571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100018289","name":"Shinnihon Foundation of Advanced Medical Treatment Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018289","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>As liquid\u2010based cytology (LBC) specimens preserve high\u2010quality DNA, clinical sequencing of LBC specimens using next\u2010generation sequencing (NGS) is becoming a common strategy. This study aimed to evaluate the feasibility of NGS\u2010based custom\u2010made panels for evaluating <jats:italic>MUC<\/jats:italic> promoter methylation in LBC specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010one patients with pancreatic cancer were enrolled in the study. Cancer tissue samples were obtained using endoscopic ultrasound\u2010guided fine\u2010needle aspiration\/biopsy (EUS\u2010FNA\/FNB). LBC, formalin\u2010fixed paraffin\u2010embedded (FFPE), and fresh frozen specimens were prepared for DNA extraction after pathological diagnosis. These specimens were then subjected to NGS analysis using custom\u2010made cancer gene screening and methylation panels comprising 28 cancer\u2010related genes and 13 gene promoter regions, including <jats:italic>MUC1<\/jats:italic>, <jats:italic>MUC2<\/jats:italic>, and <jats:italic>MUC4<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The success rate of NGS using the cancer gene panel was comparable among the LBC, FFPE, and frozen specimens, and the presence of cancer cell\u2010derived somatic mutations in each specimen was confirmed. The specimens were then tested using a methylation panel that revealed the sequential methylation status of CpG islands located in the promoter regions of <jats:italic>MUC<\/jats:italic> genes. The methylation status results obtained from LBC specimens were almost comparable with those from FFPE and frozen specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>MUC<\/jats:italic> and other gene methylation analyses using an NGS\u2010based panel were successfully performed in residual LBC specimens obtained by EUS\u2010FNA\/FNB. Therefore, this approach provides an alternative source of molecular tests for gene mutations and methylation, especially in the pancreatic cancers, which are often unresectable and unsuitable for obtaining FFPE specimens.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.25022","type":"journal-article","created":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T11:16:31Z","timestamp":1659611791000},"page":"499-507","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Sequential evaluation of <scp>MUC<\/scp> promoter methylation using next\u2010generation sequencing\u2010based custom\u2010made panels in liquid\u2010based cytology specimens of pancreatic cancer"],"prefix":"10.1002","volume":"50","author":[{"given":"Sieya","family":"Yokoyama","sequence":"first","affiliation":[{"name":"Department of Pathology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima 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