{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T03:23:28Z","timestamp":1784172208053,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T00:00:00Z","timestamp":1681948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To confirm the predictive value of targeted therapies for oncogenic driver gene mutations detected in malignant pleural effusion (MPE) cell blocks from patients with advanced non\u2010small cell lung cancer (NSCLC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>For patients with NSCLC whose tumor tissues could not be used to detect oncogenic driver gene status, molecular mutation status in 101 MPE cell blocks was tested using amplification refractory mutation system polymerase chain reaction prior to treatment. Corresponding targeted therapies were adopted based on the detection results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mutations observed in MPE cell blocks included epidermal growth factor receptor mutation (<jats:italic>EGFR<\/jats:italic>) (60.4% [61\/101]), anaplastic lymphoma kinase fusion (6.3% [5\/80]), and ROS proto\u2010oncogene 1 receptor tyrosine kinase fusion (3% [2\/70]). Other mutations that were found in &lt;5% of patients included epidermal growth factor receptor\u20102, rat sarcoma\u2010filtered germ carcinogenic homologous B1, neuroblastoma RAS viral oncogene homolog, and mesenchymal epithelial transition factor exon 14. The median follow\u2010up time was 23.5\u2009months for the 41 patients with a single <jats:italic>EGFR<\/jats:italic> mutation and who received tyrosine kinase inhibitor monotherapy as the first\u2010line treatment; in these patients, the objective response rate was 78% (95% confidence intervals (CI), 62% to 89%), progression\u2010free survival was 10.8\u2009months (95% CI, 8.7 to 13.0\u2009months), and overall survival was 31.7\u2009months (95% CI, 13.9 to 49.4\u2009months).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Malignant pleural effusion cell blocks are recommended for mutation testing for targeted therapies in patients with NSCLC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.25143","type":"journal-article","created":{"date-parts":[[2023,4,20]],"date-time":"2023-04-20T17:23:51Z","timestamp":1682011431000},"page":"397-405","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Role of 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