{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T21:58:39Z","timestamp":1788299919350,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T00:00:00Z","timestamp":1683676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>As liquid\u2010based cytology (LBC) specimens harbor high\u2010quality DNA, genomic analysis using LBC specimens is beneficial for integrative diagnosis. This study aimed to clarify the feasibility of LBC specimens for a bimodal application of DNA\u2010 and RNA\u2010based next\u2010generation sequencing (NGS) panels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>LBC specimens were prepared from cultured human cancer HEC59 cells using commercially available fixatives (Cellprep, CytoRich Red, and SurePath solutions), and were subjected to NGS for a feasibility study. Clinical LBC specimens of thyroid and salivary gland tumors were prepared using CytoRich Red solution. After DNA and RNA extraction, NGS analyses were performed in a single run using combined DNA\u2010 and RNA\u2010based custom\u2010made cancer panels for the detection of gene mutations and fusions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High\u2010quality DNA and RNA were obtained, and the expected gene mutations and fusions were detected in HEC59 cells using all types of LBC fixatives. Most available clinical cases (18 out of 20) exhibited pathogenic gene mutations (15 cases) and fusion genes (3 cases) using the bimodal DNA\u2010 and RNA\u2010based panels. Overall, 18 cases (90%) showed oncogenic mutations or fusion genes of diagnostic values.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Simultaneous application of bimodal DNA\u2010 and RNA\u2010based gene panels was useful in NGS analysis using residual LBC specimens for integrative diagnosis. Residual LBC specimens for genomic analysis, including fusion gene analysis, are particularly useful for obtaining genomic information before surgical resection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.25149","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T03:52:20Z","timestamp":1683777140000},"page":"493-500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Cancer gene analysis of liquid\u2010based cytology specimens using next\u2010generation sequencing: A technical report of bimodal <scp>DNA<\/scp>\u2010 and <scp>RNA<\/scp>\u2010based panel application"],"prefix":"10.1002","volume":"51","author":[{"given":"Toshiaki","family":"Akahane","sequence":"first","affiliation":[{"name":"Department of Pathology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"},{"name":"Center for Human Genome and Gene Analysis Kagoshima University Hospital  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