{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:51:39Z","timestamp":1747147899151,"version":"3.40.5"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T00:00:00Z","timestamp":1697155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Claudin\u20104, a tight junction associated protein expressed in epithelial cells, is purported as a highly specific and sensitive marker for epithelial malignancies. Our aim was to assess the sensitivity, specificity and real\u2010time utility of claudin\u20104 immunocytochemistry (ICC) in the diagnostic work\u2010up of suspected malignant effusions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Claudin\u20104 (3E2C1 clone) ICC was performed prospectively in effusion cell blocks where other ICC markers were being performed as part of reporting over 3\u2009months. Based on claudin\u20104 staining in unequivocal malignant and reactive effusions, the sensitivity and specificity was calculated. In cases signed out as inconclusive encompassing atypia of undetermined significance (AUS) and suspicious for malignancy (SFM) and negative for malignancy, change in diagnostic category based on addition of claudin\u20104 ICC was assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Study included 107 effusions. Claudin\u20104 stained 100% of metastatic adenocarcinomas including those with primaries in lung, breast, ovary, female genital tract, gastrointestinal tract and pancreatic\u2010biliary tract, and was negative in all reactive mesothelial and mesothelioma effusions with sensitivity of 100% (48\/48) and specificity of 95% (20\/21) for adenocarcinoma. Claudin\u20104 upgraded the diagnostic category to positive in 70% (16\/23) of SFM, 20% (1\/5) of AUS, and in 50% (5\/10) of negative effusions. Among cases with confirmed serosal involvement status on follow\u2010up, claudin\u20104 showed sensitivity, specificity, positive predictive value and negative predictive values of 85% (11\/13), 100% (3\/3), 100% (10\/10) and 75% (3\/4), respectively, for metastatic adenocarcinoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Claudin\u20104 as a single marker is sensitive and specific for adenocarcinoma and is a valuable addition to the ICC armamentarium.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.25238","type":"journal-article","created":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T06:55:08Z","timestamp":1697266508000},"page":"30-41","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Claudin\u20104 immunocytochemistry is specific and sensitive for the diagnosis of malignant carcinomatous effusions: Results from a pilot study"],"prefix":"10.1002","volume":"52","author":[{"given":"Vanlal","family":"Hruaii","sequence":"first","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5583-1720","authenticated-orcid":false,"given":"Balamurugan","family":"Thirunavukkarasu","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vindhya","family":"Prabha","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandeep","family":"Mathur","sequence":"additional","affiliation":[{"name":"Department of Pathology All India Institute of Medical Sciences  New Delhi 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