{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:51:40Z","timestamp":1747147900869,"version":"3.40.5"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T00:00:00Z","timestamp":1699401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1A2C4086635","2021R1F1A1063982"],"award-info":[{"award-number":["2021R1A2C4086635","2021R1F1A1063982"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To compare the immunochemical expression of EGFR, PD\u2010L1, and the mismatch repair (MMR) proteins MLH1, PMS2, MSH2, and MSH6 between matched malignant effusions obtained before and following the administration of chemotherapy in patients with high\u2010grade serous tubo\u2010ovarian carcinoma (HGSC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In the enrolled HGSCs, matched formalin\u2010fixed and paraffin\u2010embedded cell blocks (CBs) from effusions sampled before (treatment\u2010na\u00efve patients) and during recurrence (following chemotherapy administration), in addition to their matched HGSC tissues obtained from the ovaries at initial diagnosis (treatment\u2010na\u00efve patients), were subjected to EGFR, PD\u2010L1, and MMR immunochemical analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EGFR was more often overexpressed in effusions obtained after chemotherapy administration compared to both effusions (100% vs. 57.1%) and their matched tubo\u2010ovarian tumors (100% vs. 7.1%) from treatment\u2010na\u00efve patients, respectively. EGFR immunochemistry was concordant in just 9.1% of the effusions sampled during recurrence and their paired ovarian samples before recurrence. Whereas all HGSC treatment\u2010na\u00efve samples (ovarian lesions and effusions) were PD\u2010L1 negative, 3\/11 (27.3%) malignant effusions obtained during recurrence showed PD\u2010L1 overexpression. Lastly, none of the tested HGSC samples exhibited MMR deficiency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Measuring biomarkers using CBs from malignant effusions may provide clinicians with significant information related to HGSC prognosis and therapy selection, especially in patients with resistance to chemotherapy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.25248","type":"journal-article","created":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T08:27:25Z","timestamp":1699432045000},"page":"69-75","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression of <scp>EGFR<\/scp>, <scp>PD\u2010L1<\/scp>, and the mismatch repair proteins before and following therapy in malignant serous effusions with metastatic high\u2010grade serous tubo\u2010ovarian carcinoma"],"prefix":"10.1002","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8625-2556","authenticated-orcid":false,"given":"Ilias P.","family":"Nikas","sequence":"first","affiliation":[{"name":"School of Medicine European University Cyprus  Nicosia Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo\u2010Young","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Pathology Seoul National University Hospital  Seoul Republic of Korea"},{"name":"Department of Pathology Seoul National University College of Medicine  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Ji","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Pathology Seoul National University Hospital  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheol","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pathology Seoul 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