{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:36:34Z","timestamp":1788280594833,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T00:00:00Z","timestamp":1699920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2019JJ80036"],"award-info":[{"award-number":["2019JJ80036"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Ultrasound\u2010guided fine\u2010needle aspiration cytology (FNAC) is a routine preoperative method for evaluating suspicious axillary lymph nodes (ALNs) in patients with breast cancer. However, a range of reasons such as morphological pitfalls, technical artifacts, and sampling errors restrict the sensitivity and accuracy of FNAC. This retrospective study investigated the diagnostic value of GATA\u2010binding protein 3 (GATA\u20103) immunocytochemistry for FNAC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Breast cancer patients who underwent preoperative FNAC for suspicious ALNs, relevant GATA\u20103 immunocytochemistry, and postoperative status of ALNs were reviewed from the period of March 2020 to February 2022. Altogether, 102 patients were included in the study. FNAC material smears stained with hematoxylin and eosin was initially assessed by two cytopathologists and categorized into five groups: nondiagnostic, negative, atypical, suspicious, and positive for malignancy. Only group of cells positive for malignancy was considered positive. For each case, two selected slides were digitized (whole slide imaged) at \u00d740 magnification and decolored for GATA\u20103 immunocytochemistry. The expression of GATA\u20103 was scored ranging from 0 to 9 (Score \u22653: Positive, Score \u22642: Negative). If either FNAC or GATA\u20103 immunocytochemistry was positive or the combined test positive, then the case was considered positive. The sensitivity, specificity, and accuracy of FNAC, GATA\u20103 immunocytochemistry, and combined FNAC\/GATA\u20103 immunocytochemistry were analyzed by <jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> and Fisher's tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean age of the study participants was 50.62 (ranging: 30\u201373\u2009years). Invasive breast carcinoma (not otherwise specified) accounted for most histological subtypes, and grade 2 was the leading Nottingham grade. Sixteen cases directly underwent mastectomy while the other 86 patients had neoadjuvant therapy. A more serious diagnosis was made based on GATA\u20103 detection in 22.5% (<jats:italic>n<\/jats:italic>\u2009=\u200923) of 102 cases. Of the 23 cases, metastasis was confirmed by GATA\u20103 detection in 21 cases, and an uncertain diagnosis was ascertained based on GATA\u20103 immunocytochemistry in 2 with nondiagnostic FNAC results. The sensitivity (77\/87, 88.5%) of GATA\u20103 detection for distinguishing malignancies from benign lesions was higher than that of FNAC alone (62\/87, 71.3%) (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>GATA\u20103 immunocytochemistry exhibited high diagnostic efficacy in distinguishing malignant breast cancer cells. Moreover, combined FNAC and GATA\u20103 immunocytochemistry achieved optimal results in terms of reducing the false\u2010negative rate and promoting accuracy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.25252","type":"journal-article","created":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T20:43:32Z","timestamp":1700081012000},"page":"93-102","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Utility of <scp>GATA<\/scp>\u20103 immunocytochemistry for the assessment of fine\u2010needle aspiration in breast cancer patients with suspicious axillary lymph nodes at ultrasound"],"prefix":"10.1002","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9097-1841","authenticated-orcid":false,"given":"Zhihua","family":"Lan","sequence":"first","affiliation":[{"name":"Department of Pathology, The First Affiliated Hospital, Hengyang Medical School University of South China  Hengyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, The First Affiliated Hospital, Hengyang Medical School University of South China  Hengyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology, The First Affiliated Hospital, Hengyang Medical School University of South China  Hengyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongfang","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pathology, The First Affiliated Hospital, Hengyang Medical School University of South China  Hengyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.10920"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41523-021-00298-6"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2011.90"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12957-019-1582-z"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3747\/co.20.1294"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12022"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21575"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-40811-3"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbj.13402"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2022.04.006"},{"issue":"2","key":"e_1_2_9_12_1","first-page":"242","article-title":"Diagnostic accuracy of us\u2010Fnac of axillary lymph nodes in patients with primary breast cancer using sentinel lymph node biopsy as standard reference","volume":"31","author":"Fayyaz MB","year":"2019","journal-title":"J Ayub Med Coll Abbottabad"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-018-4062-x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctro.2021.12.010"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-017-6090-1"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.13122637"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.7863\/ultra.32.12.2177"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000440797"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21384"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2016.01.008"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.21153"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics10090717"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-00697-3"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21393"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21574"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6600744"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.7863\/ultra.15.06019"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-016-3755-6"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-00692-8"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22623"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.25252","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T05:19:28Z","timestamp":1705900768000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.25252"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,14]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/dc.25252"],"URL":"https:\/\/doi.org\/10.1002\/dc.25252","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,14]]},"assertion":[{"value":"2023-07-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}