{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T08:56:02Z","timestamp":1765961762924,"version":"3.40.5"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:00:00Z","timestamp":1705968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Programmed death\u2010ligand 1 (PD\u2010L1) expression levels measured by immunohistochemistry have been proven to predict the outcome of immunotherapy in lung adenocarcinoma (LUAD). However, data on PD\u2010L1 expression on liquid\u2010based cytology (LBC) in malignant pleural effusion (MPE) is scarce.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study cohort included 60 cases with MPE suffering from LUAD. PD\u2010L1 SP263 assay was used for immunocytochemistry (ICC) on LBC and matched cell block (CB) to validate ICC protocols on LBC slides. Clinical outcomes were analyzed based on immunotherapy and PD\u2010L1 tumor proportion scores (TPS) on LBC slides and CBs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PD\u2010L1 expression with TPS \u22651% was lower in LBCs than in CBs (33 of 60 [55.0%] vs. 35 of 60 [58.3%]; <jats:italic>p<\/jats:italic>\u2009=\u2009.687). Even with the TPS \u226550% threshold, PD\u2010L1 expression was lower in LBCs (10 of 60 [16.7%] vs. 15 of 60 [25%]; <jats:italic>p<\/jats:italic>\u2009=\u2009.125). Epidermal growth factor receptor (EGFR) exon 20 mutation, tumor cell proportion, and pleural fluid neutrophil\u2010to\u2010lymphocyte ratio were related to PD\u2010L1 expression on CBs (<jats:italic>p<\/jats:italic>\u2009=\u2009.013, <jats:italic>p<\/jats:italic>\u2009=\u20090.022, and <jats:italic>p<\/jats:italic>\u2009=\u2009.011), respectively. Patients with subsequent immune checkpoint inhibitor therapy remained a better prognostic in subgroups of PD\u2010L1 positive expression on LBC slides (TPS \u22651%, <jats:italic>p<\/jats:italic>\u2009=\u2009.041).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>LBC specimens had comparable performance to CBs in PD\u2010L1 assessment and predicting treatment response to PD\u2010L1\u2010defined therapy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.25276","type":"journal-article","created":{"date-parts":[[2024,1,24]],"date-time":"2024-01-24T07:15:36Z","timestamp":1706080536000},"page":"235-242","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The detection of <scp>PD\u2010L1<\/scp> expression on <scp>liquid\u2010based<\/scp> cytology in pleural effusion of lung adenocarcinoma and its prognostic evaluation: Between paired <scp>liquid\u2010based<\/scp> cytology and cell block samples"],"prefix":"10.1002","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8155-9057","authenticated-orcid":false,"given":"Haiyue","family":"Ma","sequence":"first","affiliation":[{"name":"Cytopathology Section, Department of Pathology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Jia","sequence":"additional","affiliation":[{"name":"Cytopathology Section, Department of Pathology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zihan","family":"Sun","sequence":"additional","affiliation":[{"name":"Cytopathology Section, Department of Pathology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyue","family":"Xiao","sequence":"additional","affiliation":[{"name":"Cytopathology Section, Department of Pathology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuo","family":"Liang","sequence":"additional","affiliation":[{"name":"Cytopathology Section, Department of Pathology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linlin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Cytopathology Section, 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