{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:52:55Z","timestamp":1787503975070,"version":"build-2736575974"},"reference-count":21,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T00:00:00Z","timestamp":1714089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100014434","name":"Irving Medical Center, Columbia University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Dual immunostaining for p16\/Ki67 is FDA\u2010approved for use on liquid\u2010based cervical cytology specimens; however, the utility of dual staining in anal cytology especially for ASCUS risk stratification is not well established.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We investigated dual staining performance on anal cytology specimens and correlated with subsequent cytologic interpretation, high\u2010risk HPV status, and anal biopsy results. Dual staining for p16\/Ki\u201067 was performed on all liquid\u2010based anal cytology specimens from December 2021 to June 2022 (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200943).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Three patients had high grade squamous intraepithelial lesion (HSIL\/AIN2\u20103) on biopsy; dual staining was positive in all three cases. All HR\u2010HPV negative cases were negative for dual staining. Among the 12 ASCUS samples with subsequent anal biopsy results all also had HR\u2010HPV testing. Due to small sample size of cases with squamous intraepithelial lesion (SIL) diagnosed on biopsy, the sensitivity and positive predictive value was not calculated. However, the specificity and negative predictive value of p16\/Ki\u201067 dual staining for SIL of any grade on biopsy were 1 (95% CI: 0.66\u20131) and 0.9 (95% CI: 0.65\u20130.97) respectively, whereas the specificity and negative predictive value of HR\u2010HPV testing for SIL of any grade on biopsy were 0.44 (95% CI: 0.14\u20130.79) and 0.8 (95% CI: 0.41\u20130.96) respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Dual p16\/Ki\u201067 staining indicates transforming HPV infection and could help serve as an ancillary test for risk stratification for atypical anal cytology specimens. Among ASCUS samples, dual staining was specific for SIL of any grade with a high negative predictive value and therefore could be useful in clinical practices with limited availability for follow\u2010up care.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dc.25332","type":"journal-article","created":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T04:53:55Z","timestamp":1714107235000},"page":"469-474","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of dual p16\/Ki\u201067 immunostaining on anal cytology specimens"],"prefix":"10.1002","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6706-0700","authenticated-orcid":false,"given":"Marie C.","family":"Smithgall","sequence":"first","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Irving Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William S.","family":"Towne","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Irving Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abel A.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Irving Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adela","family":"Cimic","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology Columbia University Irving Medical Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/curroncol30030246"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.34850"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2008.034744"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22003"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22151"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20126"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-11074-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasc.2015.06.004"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2017\u20100242\u2010RA"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiaa801"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2201048"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3233\/CBM-210366"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33250"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33748"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181f0f52a"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.34199"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.31557\/APJCP.2020.21.7.2013"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e328359f255"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0188851"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.4103\/JOC.JOC_131_18"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10070888"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.25332","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T13:35:23Z","timestamp":1758548123000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.25332"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,26]]},"references-count":21,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/dc.25332"],"URL":"https:\/\/doi.org\/10.1002\/dc.25332","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,26]]},"assertion":[{"value":"2024-01-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}