{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T16:49:56Z","timestamp":1783529396292,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T00:00:00Z","timestamp":1733097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The value of lymphocyte profiling (LP) in mediastinal lymph nodes for the differential diagnosis of sarcoidosis has not been extensively studied, and existing literature presents mixed results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This was a prospective study of patients with intrathoracic lymphadenopathy who underwent endobronchial ultrasound\u2010guided transbronchial needle aspiration (EBUS\u2010TBNA). LP in lymph node puncture fluid (LNPF) was evaluated using flow cytometry. The results of LP in sarcoidosis patients were compared with tuberculous lymphadenitis (TBLA) patients. Receiver operating characteristic (ROC) analysis was performed to determine the optimal cut\u2010offs of the statistically significant parameters for screening for sarcoidosis. Based on the optimal cut\u2010offs and the final diagnosis of sarcoidosis and TBLA, the sensitivity, specificity, and accuracy of every statistically significant parameter and different combinations of the above three parameters were calculated for the diagnosis of sarcoidosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Forty\u2010five cases of sarcoidosis and 33 cases of TBLA were enrolled in this study. Compared with the LP in TBLA patients, in sarcoidosis patients, the proportion of CD4 T cells and CD4\/CD8 ratio increased, and the proportion of CD8 T cells and natural killer (NK) cells decreased. Among all single parameters, the CD4\/CD8 ratio had high diagnostic sensitivity (84.4%), specificity (81.8%), and accuracy (83.3%) for sarcoidosis. Among all the combinations of three parameters, the combination of CD4, CD8, and NKT\/NK ratio had high diagnostic sensitivity (91.1%), specificity (84.8%), and accuracy (87.2%) for sarcoidosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Assessment of LP in LNPF may improve the differential diagnostic accuracy of sarcoidosis from TBLA and further strengthen the importance of LP in LNPF in the diagnostic workup of sarcoidosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dc.25418","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T07:43:13Z","timestamp":1733211793000},"page":"83-90","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Role of the Lymphocyte Profile in Mediastinal Lymph Nodes in the Differential Diagnosis of Sarcoidosis and Tuberculous Lymphadenitis Patients Undergoing <scp>EBUS<\/scp>\u2010<scp>TBNA<\/scp>"],"prefix":"10.1002","volume":"53","author":[{"given":"Song","family":"Mi","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1869-3744","authenticated-orcid":false,"given":"Kewu","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao\u2010Yang Hospital Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,2]]},"reference":[{"issue":"5","key":"e_1_2_11_2_1","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1164\/rccm.201006-0865CI","article-title":"A Concise Review of Pulmonarysarcoidosis","volume":"183","author":"Baughman R. P.","year":"2011","journal-title":"American Journal of Respiratory and Critical Care Medicine"},{"issue":"5","key":"e_1_2_11_3_1","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1097\/MCP.0b013e3283560809","article-title":"Sarcoidosis and Tuberculosis: The Same Disease With Different Manifestations or Similar Manifestations of Different Disorders","volume":"18","author":"Gupta D.","year":"2012","journal-title":"Current Opinion in Pulmonary Medicine"},{"key":"e_1_2_11_4_1","first-page":"50","article-title":"Difficulties in Managing Lymph Node Tuberculosis","volume":"21","author":"Gupta P. R.","year":"2004","journal-title":"Lung India"},{"issue":"2","key":"e_1_2_11_5_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0149258","article-title":"Genotypic, Phenotypic and Clinical Validation of GeneXpert in Extra\u2010Pulmonary and Pulmonary Tuberculosis in India","volume":"11","author":"Singh U. 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L.","year":"1993","journal-title":"Journal of Experimental Medicine"},{"issue":"4","key":"e_1_2_11_32_1","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1038\/mi.2017.15","article-title":"Novel Role for IL\u201022 in Protection During Chronic Mycobacterium Tuberculosis HN878 Infection","volume":"10","author":"Treerat P.","year":"2017","journal-title":"Mucosal Immunology"},{"issue":"4","key":"e_1_2_11_33_1","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1007\/s10238-019-00578-3","article-title":"NK and NKT\u2010Like Cells in Granulomatous and Fibrotic Lung Diseases","volume":"19","author":"Bergantini L.","year":"2019","journal-title":"Clinical and Experimental Medicine"},{"issue":"1","key":"e_1_2_11_34_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s12890-019-0802-1","article-title":"NK and NKT Cells in the Diagnosis of Diffuse Lung Diseases Presenting With a Lymphocytic Alveolitis","volume":"19","author":"Sokhatska O.","year":"2019","journal-title":"BMC Pulmonary Medicine"},{"issue":"10","key":"e_1_2_11_35_1","doi-asserted-by":"crossref","first-page":"7086","DOI":"10.4049\/jimmunol.177.10.7086","article-title":"NK Cell\u2010Derived IFN\u2010Gamma Differentially Regulates Innate Resistance and Neutrophil Response in T Cell\u2010Deficient Hosts Infected With Mycobacterium Tuberculosis","volume":"177","author":"Feng C. G.","year":"2006","journal-title":"Journal of Immunology"},{"issue":"2","key":"e_1_2_11_36_1","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1006\/cimm.1994.1196","article-title":"Interleukin\u201012 (IL\u201012) Augments Cytolytic Activity of Natural Killer Cells Toward Mycobacterium Tuberculosis\u2010Infected Human Monocytes","volume":"156","author":"Denis M.","year":"1994","journal-title":"Cellular Immunology"},{"issue":"1","key":"e_1_2_11_37_1","doi-asserted-by":"crossref","first-page":"130","DOI":"10.4049\/jimmunol.172.1.130","article-title":"NK Cells Regulate CD8+ T Cell Effector Function in Response to an Intracellular Pathogen","volume":"172","author":"Vankayalapati R.","year":"2004","journal-title":"Journal of Immunology"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.25418","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T09:41:08Z","timestamp":1735724468000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.25418"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,2]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/dc.25418"],"URL":"https:\/\/doi.org\/10.1002\/dc.25418","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,2]]},"assertion":[{"value":"2024-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}