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Aspiration biopsy material from 55 benign and malignant breast lesions was smeared onto slides and stained with a routine Papanicolaou technique (after wet fixation) or with the Azur B\u2010eosin Y stain (after air drying). Tumors were grouped according to both histological grade and lymph node status. Nuclear area, diameter, and perimeter were measured from each specimen with an image analyzer and correlated to grade and stage of the disease. All nuclear parameters were significantly smaller in wet\u2010fixed slides. The coefficient of variation was smaller in air\u2010dried samples, which allowed better discrimination between different groups. Nuclear perimeter had the highest discriminative power. Unintentional air drying in wet\u2010fixed smears increased the coefficient of variation for all nuclear parameters. Air drying of cytologic smears is recommended for morphometric investigations if chromatin texture is of minor importance.<\/jats:p>","DOI":"10.1002\/dc.2840030316","type":"journal-article","created":{"date-parts":[[2007,2,22]],"date-time":"2007-02-22T01:41:07Z","timestamp":1172108467000},"page":"256-261","source":"Crossref","is-referenced-by-count":14,"title":["The influence of the wet\u2010fixed papanicolaou and the air\u2010dried giemsa techniques on nuclear parameters in breast cancer cytology: A cytomorphometric study"],"prefix":"10.1002","volume":"3","author":[{"given":"E.","family":"Schulte","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chr.","family":"Wittekind","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,12]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"313","article-title":"Evaluation of needle aspirates and tissue sections as prognostic factors in mammary carcinoma","volume":"20","author":"Wallgren A","year":"1976","journal-title":"Acta Cytol"},{"key":"e_1_2_1_3_2","first-page":"332","article-title":"Needle cytology of the breast: a quantitative study of the cells of benign and malignant ductal neoplasia","volume":"23","author":"Dziura DR","year":"1979","journal-title":"Acta Cytol"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197910)44:4<1458::AID-CNCR2820440440>3.0.CO;2-T"},{"key":"e_1_2_1_5_2","first-page":"787","article-title":"Studies on cytologic characterization of mammary aspiration smears based on histologic types","volume":"26","author":"Takeda T","year":"1982","journal-title":"Acta Cytol"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19830401)51:7<1182::AID-CNCR2820510703>3.0.CO;2-A"},{"key":"e_1_2_1_7_2","first-page":"20","volume-title":"Major problems in pathology","author":"Frable WJ","year":"1983"},{"key":"e_1_2_1_8_2","first-page":"479","article-title":"The prognostic value of aspiration biopsy smear in mammary carcinoma","volume":"20","author":"Wallgren A","year":"1976","journal-title":"Acta Cytol"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.39.5.489"},{"key":"e_1_2_1_10_2","first-page":"75","article-title":"The relation of prognosis to the nuclear diameter of breast cancer cells obtained by cytologic aspiration","volume":"23","author":"Zajdela A","year":"1979","journal-title":"Acta Cytol"},{"key":"e_1_2_1_11_2","first-page":"128","article-title":"Quantitative analysis of the nuclear area variation in benign and malignant breast cytology specimens","volume":"3","author":"Cornelisse CJ","year":"1981","journal-title":"Acta Cytol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1982.tb02727.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(82)80004-X"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19850715)56:2<374::AID-CNCR2820560229>3.0.CO;2-9"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1982.170"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19821215)50:12<2884::AID-CNCR2820501231>3.0.CO;2-K"},{"key":"e_1_2_1_17_2","first-page":"253","article-title":"Automated microscope image analysis in the cytologic tumor diagnostics. 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