{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:10:38Z","timestamp":1789171838713,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Molecular classification of endometrial cancer is useful for predicting prognosis. Genomic examinations are performed using formalin\u2010fixed paraffin\u2010embedded (FFPE) tissues; however, we previously reported that liquid\u2010based cytology (LBC) specimens can be used for next\u2010generation sequencing (NGS). In this study, we evaluated long\u2010term storage effects of LBC specimens on NGS\u2010based genomic profiling, including gene mutations, tumor mutation burden (TMB), and microsatellite instability (MSI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Four LBC fixatives (CellPrep, ThinPrep, CytoRich Red, and SurePath) were used to prepare NGS samples from cultured endometrioid carcinoma HEK\u2010251 cells. Twelve endometrial LBC specimens from patients with endometrioid carcinoma were fixed with CytoRich Red. The TMB, MSI, and gene mutations were analyzed after 1\u2009week, 6\u2009months, and 12\u2009months of storage in cultured HEK\u2010251 cells. Paired LBC and FFPE specimens of endometrioid carcinoma stored for 15\u201345\u2009months were subjected to NGS\u2010based analysis, and their molecular profiles were compared to those at the initial diagnosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The TMB and MSI did not differ during the storage periods for any of the LBC fixatives in the cultured cells; in addition to gene mutations, they were comparable between the initial and second analyses of the clinical FFPE and LBC specimens. There were no changes in the integrative diagnosis of the endometrioid carcinoma subtype classification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>LBC specimens, which preserved high\u2010quality genomes for molecular classification after long\u2010term storage, may be an alternative source of genomic examination for the integrative diagnosis of endometrial cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dc.70008","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T10:30:26Z","timestamp":1755513026000},"page":"536-545","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Endometrial Liquid\u2010Based Cytology Specimens Preserve High Genome Quality for Molecular Classification After Long\u2010Term Storage"],"prefix":"10.1002","volume":"53","author":[{"given":"Toshiaki","family":"Akahane","sequence":"first","affiliation":[{"name":"Department of Pathology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"},{"name":"Center for Human Genome and Gene Analysis Kagoshima University Hospital  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6746-6695","authenticated-orcid":false,"given":"Ikumi","family":"Kitazono","sequence":"additional","affiliation":[{"name":"Unit of Surgical Pathology Kagoshima University Hospital  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiya","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Department of Pathology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuriko","family":"Higashi","sequence":"additional","affiliation":[{"name":"Department of Pathology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"},{"name":"Department of Obstetrics and Gynecology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7508-1624","authenticated-orcid":false,"given":"Akihide","family":"Tanimoto","sequence":"additional","affiliation":[{"name":"Department of Pathology Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"},{"name":"Center for Human Genome and Gene Analysis Kagoshima University Hospital  Kagoshima Japan"},{"name":"Unit of Surgical Pathology Kagoshima University Hospital  Kagoshima Japan"},{"name":"Center for the Research of Advanced Diagnosis and Therapy of Cancer Kagoshima University Graduate School of Medical and Dental Sciences  Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21561"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.22807"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24410"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24956"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0217724"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21812"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21897"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22098"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24511"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12920\u2010020\u201000753\u20106"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.25149"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.25022"},{"key":"e_1_2_9_14_1","first-page":"245","volume-title":"Female Genital Tumours","author":"Kim K. R.","year":"2020"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12113"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24841"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/pore.2021.1610013"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2023.154563"},{"issue":"3","key":"e_1_2_9_19_1","first-page":"373","article-title":"Endocyte Endometrial Smears in the Cytodiagnosis of Endometrial Carcinoma","volume":"34","author":"Byrne A. J.","year":"1990","journal-title":"Acta Cytologica"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.21734"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty790"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt755"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885\u2010019\u20106227\u20107"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0308985"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.13442"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics13061078"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22525"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.4103\/joc.joc_13_22"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.06.010"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428\u2010017\u20102213\u20100"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0176280"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.12631"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.13170"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.70008","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dc.70008","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.70008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:30:36Z","timestamp":1789169436000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.70008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,18]]},"references-count":32,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/dc.70008"],"URL":"https:\/\/doi.org\/10.1002\/dc.70008","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,18]]},"assertion":[{"value":"2025-02-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}