{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T02:26:03Z","timestamp":1771035963362,"version":"3.50.1"},"reference-count":9,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T00:00:00Z","timestamp":1761350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Molecular testing on FNA rinses offers a simple, minimally invasive, and cost\u2010effective alternative to tissue biopsies. Among clinically relevant biomarkers,\n                    <jats:italic>KRAS<\/jats:italic>\n                    mutations are key in guiding targeted therapy across several cancer types. The fully automated Idylla platform provides rapid analysis directly from FFPE samples, without DNA extraction. In this study, we assessed the feasibility of applying the Idylla\n                    <jats:italic>KRAS<\/jats:italic>\n                    Mutation Test to FNA needle rinses collected in saline and Cytolyt. We analyzed 30 FNA rinses from resection specimens of 27 colorectal adenocarcinomas and three pancreatic adenocarcinomas (simulated FNAs). All 30 rinses were collected in saline, and 18 were additionally collected in Cytolyt without Preservcyt transfer. After cytospin preparation to assess cellularity and tumor percentage, the rinse was centrifuged at 2500\u2009rpm for 5\u2009min to obtain a pellet. A 50\u2009\u03bcL aliquot of the pellet was directly loaded into the Idylla cartridge. Results from all rinses were compared with the Idylla\n                    <jats:italic>KRAS<\/jats:italic>\n                    Mutation Test on the corresponding FFPE tumor tissue.\n                    <jats:italic>KRAS<\/jats:italic>\n                    mutations were consistently detected in FNA rinses, with no false negatives or false positives observed. A single case, a saline rinse, was misclassified by the automated variant caller (reported as Q61K instead of the true G12D). However, amplification curve review confirmed a clear G12D, while the Q61K signal was aberrant. The Idylla\n                    <jats:italic>KRAS<\/jats:italic>\n                    Mutation Test can be reliably applied to FNA needle rinses in both saline and Cytolyt, yielding robust results in samples with high tumor content. Nevertheless, careful review of amplification curve patterns is essential in this setting.\n                  <\/jats:p>","DOI":"10.1002\/dc.70039","type":"journal-article","created":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T07:10:33Z","timestamp":1761376233000},"page":"87-93","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Validation of the Idylla\n                    <scp>\n                      <i>KRAS<\/i>\n                    <\/scp>\n                    Mutation Test on Fine\u2010Needle Aspiration Needle Rinses in Saline and Cytolyt Solution Without\n                    <scp>DNA<\/scp>\n                    Extraction: A Comparison With Paired\n                    <scp>FFPE<\/scp>\n                    Tumor Samples"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0913-4083","authenticated-orcid":false,"given":"Aida","family":"Molero","sequence":"first","affiliation":[{"name":"Pathology Department Hospital Universitario R\u00edo Hortega  Valladolid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar Ortega de la","family":"Obra","sequence":"additional","affiliation":[{"name":"Pathology Department Hospital General de Segovia  Segovia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica","family":"Mate","sequence":"additional","affiliation":[{"name":"Pathology Department Hospital General de Segovia  Segovia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Purificaci\u00f3n","family":"Manzanares","sequence":"additional","affiliation":[{"name":"Pathology Department Hospital General de Segovia  Segovia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,25]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2022.10.003"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2023.08.009"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2022.12.009"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2019.05.007"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes14081551"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24897"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24378"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2022.152023"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21666"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.70039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T09:03:29Z","timestamp":1767258209000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.70039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,25]]},"references-count":9,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/dc.70039"],"URL":"https:\/\/doi.org\/10.1002\/dc.70039","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,25]]},"assertion":[{"value":"2025-08-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}