{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T10:34:12Z","timestamp":1770892452169,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T00:00:00Z","timestamp":1765584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T00:00:00Z","timestamp":1765584000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Folate receptor\u2010\u237a (FR\u2010\u237a) is an attractive target for targeted therapy. Mirvetuximab soravtansine (MIRV) has been approved by the United States Food and Drug Administration (FDA) for treating adult patients with FR\u2010\u237a positive, platinum\u2010resistant epithelial ovarian cancer, fallopian tube cancer, or primary peritoneal cancer. Therefore, identifying and detecting FR\u2010\u237a has become an essential part of precision medicine. This study aimed to evaluate the feasibility of detecting FR\u2010\u237a protein expression in serous cavity effusions using cell blocks (CBs) and compare the results with surgical pathology biopsy or resection specimens. Furthermore, we investigated potential variations in FR\u2010\u237a expression pre\u2010 and post\u2010chemotherapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immunohistochemical (IHC) staining of FR\u2010\u237a was performed on the serous cavity effusion specimens from 35 patients with epithelial ovarian cancer, along with matched surgical pathological (SP) biopsy or resection specimens, following the manufacturer's guidelines for IHC analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Positive staining was observed in 30 (85.7%) and 32 (91.4%) serous cavity effusion CB and tissue samples, respectively. A total of 31 (88.5%) tissue and 28 (80%) effusion CB samples exhibited moderate\u2010to\u2010strong FR\u2010\u237a expression in at least 25% of tumor cells. A cutoff of 25% for FR\u2010\u237a expression positivity was used to demonstrate high concordance (96.4%) between cytology CBs and tissue specimens. We recommend a cutoff of 75% viable tumor cells with moderate\u2010to\u2010strong membrane staining as \u201cpositive\u201d in CBs for greater reliability, which aligns with FDA\u2010approved criteria. A reasonable consistency was observed in FR\u2010\u237a expression between the pre\u2010treatment serous cavity effusion CB specimens and the new biopsy specimens obtained after multiline treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We demonstrated that serous effusion CB specimens may be effective for FR\u2010\u237a biomarker detection; FR\u2010\u237a maintains stable expression pre\u2010 and post\u2010chemotherapy, and patients can benefit from MIRV.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dc.70067","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T09:20:48Z","timestamp":1765617648000},"page":"197-205","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detecting\n                    <scp>FR<\/scp>\n                    \u2010\u237a Expression Level in Cytology Effusion Specimens From Ovarian Cancer and Comparing It With Tissue Specimens"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2503-8710","authenticated-orcid":false,"given":"Yadan","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Pathology, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine Zhejiang University  Yiwu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2892-9238","authenticated-orcid":false,"given":"Xiaofei","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pathology The First People's Hospital of Yongkang  Yongkang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2973-1981","authenticated-orcid":false,"given":"Xiaohong","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine Zhejiang University  Yiwu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7885-4441","authenticated-orcid":false,"given":"Siqian","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine Zhejiang University  Yiwu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8969-3633","authenticated-orcid":false,"given":"Sishi","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine Zhejiang University  Yiwu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4171-1269","authenticated-orcid":false,"given":"Baizhou","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine Zhejiang University  Yiwu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,13]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2011.11.010"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2309169"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9651"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-020-0339-5"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25021046"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1254532"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2013.03.024"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-015-0156-0"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-23-0991"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737140.2023.2236793"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-023-04575-y"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000440796"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23448"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.519"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2023.07.010"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21850"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2007.11.020"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22688"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21937"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2007.05.018"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2017.08.015"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-014-9539-8"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.15386\/mpr-1725"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.5152\/balkanmedj.2021.20086"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12795"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.575"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-022-02031-x"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.3109\/00313025.2010.493862"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2018.04.005"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2017-204946"},{"issue":"4","key":"e_1_2_13_32_1","first-page":"279","article-title":"Immunohistochemical Detection of Estrogen Receptor, Progesterone Receptor, and Human Epidermal Growth Factor Receptor 2 Expression in Breast Carcinomas: Comparison on Cell Block, Needle\u2010Core, and Tissue Block Preparations","volume":"117","author":"Hanley K. Z.","year":"2009","journal-title":"Cancer"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.25259\/Cytojournal_56_2023"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-016-2637-y"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.70067","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dc.70067","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.70067","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T09:43:38Z","timestamp":1770889418000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.70067"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,13]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1002\/dc.70067"],"URL":"https:\/\/doi.org\/10.1002\/dc.70067","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,13]]},"assertion":[{"value":"2025-07-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}