{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T14:29:35Z","timestamp":1780324175176,"version":"3.54.1"},"reference-count":14,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Ocular surface squamous neoplasia (OSSN) presents diagnostic challenges in resource\u2010limited rural settings where access to histopathology is constrained. This exploratory study evaluates the feasibility and clinical utility of impression cytology (IC) as a non\u2010invasive diagnostic approach for OSSN in a rural Indian tertiary care center.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We conducted a retrospective analysis of seven patients with clinically suspected OSSN. IC was performed using cellulose acetate filter paper (0.22\u2009\u03bcm pore size) applied to the lesion surface for 5\u201310\u2009s. For each lesion, separate samples were collected: one fixed immediately in 95% ethanol for Hematoxylin and Eosin (H&E) staining, and one air\u2010dried followed by methanol fixation for May\u2010Gr\u00fcnwald\u2010Giemsa (MGG) staining. Cytological assessment focused on cellular atypia, nuclear\u2010cytoplasmic ratio, pleomorphism, and chromatin pattern. Clinical management and outcomes were correlated with IC findings.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n IC provided adequate cellular samples in all cases, enabling cytological grading across the OSSN spectrum: mild dysplasia (3 cases), high\u2010grade dysplasia (3 cases), and cytological features suspicious for possible invasive squamous cell carcinoma (1 case). In cases treated with topical mitomycin C (MMC) based on IC guidance, complete lesion resolution was achieved at last follow\u2010up (ranging from 3 to 6\u2009months) without recurrence. Histopathological confirmation was available in 3 cases (including the case suspicious for invasion) and showed complete concordance with IC findings. The procedure was well\u2010tolerated with 100% patient compliance.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Impression cytology is a feasible, minimally invasive diagnostic tool for OSSN that can identify dysplastic changes and guide clinical management in resource\u2010limited settings. While it cannot definitively diagnose stromal invasion and histopathological confirmation remains necessary when invasion is suspected, IC serves as a useful screening and monitoring modality in rural healthcare environments. Larger prospective studies are needed to validate these preliminary findings.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/dc.70121","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T06:41:14Z","timestamp":1775630474000},"page":"475-485","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impression Cytology as a Diagnostic Tool for Ocular Surface Squamous Neoplasia: An Exploratory Case Series From a Rural Tertiary Care Center in India"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2412-9853","authenticated-orcid":false,"given":"Arti","family":"Agarwal","sequence":"first","affiliation":[{"name":"Department of Pathology Uttar Pradesh University of Medical Sciences Etawah Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2198-9132","authenticated-orcid":false,"given":"Sanjeev Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pathology Uttar Pradesh University of Medical Sciences Etawah Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4919-3443","authenticated-orcid":false,"given":"Roopak","family":"Aggarwal","sequence":"additional","affiliation":[{"name":"Department of Pathology Uttar Pradesh University of Medical Sciences Etawah Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saniya Zehra","family":"Rizvi","sequence":"additional","affiliation":[{"name":"Department of Pathology Uttar Pradesh University of Medical Sciences Etawah Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2292-7774","authenticated-orcid":false,"given":"Deepti","family":"Joshi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Uttar Pradesh University of Medical Sciences Etawah Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8981-607X","authenticated-orcid":false,"given":"Anita","family":"Omhare","sequence":"additional","affiliation":[{"name":"Department of Pathology Government Medical College Kannauj Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5739-4241","authenticated-orcid":false,"given":"Nidhi","family":"Yaduvanshi","sequence":"additional","affiliation":[{"name":"Department of Microbiology Uttar Pradesh University of Medical Sciences Etawah Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,7]]},"reference":[{"issue":"4","key":"e_1_2_11_2_1","first-page":"480","article-title":"Ocular Surface Squamous Neoplasia","volume":"63","author":"Lee G. 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R.","year":"1977","journal-title":"Archives of Ophthalmology"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2005.073916"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(85)33967-2"},{"issue":"5","key":"e_1_2_11_10_1","first-page":"725","article-title":"Conjunctival Goblet Cell Densities in Ocular Surface Disease","volume":"107","author":"Nelson J. D.","year":"1989","journal-title":"Archives of Ophthalmology"},{"issue":"1","key":"e_1_2_11_11_1","first-page":"12","article-title":"Impression Cytology in the Evaluation of Ocular Surface Squamous Neoplasia\u2014A Retrospective Cohort Study","volume":"41","author":"Patil M.","year":"2024","journal-title":"Journal of Cytology"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/jpbs.jpbs_114_22"},{"issue":"4","key":"e_1_2_11_13_1","first-page":"289","article-title":"Update on Ocular Surface Squamous Neoplasia","volume":"31","author":"Napora C.","year":"2020","journal-title":"Current Opinion in Ophthalmology"},{"key":"e_1_2_11_14_1","volume-title":"World Health Report: Primary Health Care","author":"World Health Organization","year":"2018"},{"issue":"3","key":"e_1_2_11_15_1","first-page":"334","article-title":"Clinicopathologic Correlation of Ocular Surface Squamous Neoplasia: A Single\u2010Center Experience","volume":"38","author":"Kao A. A.","year":"2019","journal-title":"Cornea"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.70121","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dc.70121","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.70121","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T13:50:35Z","timestamp":1780321835000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.70121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,7]]},"references-count":14,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/dc.70121"],"URL":"https:\/\/doi.org\/10.1002\/dc.70121","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,7]]},"assertion":[{"value":"2026-02-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}