{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:23:34Z","timestamp":1788276214138,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Anal cancer rates are rising among women, immunocompromised individuals, and Men who have sex with Men (MSM), independent of HIV status. While high\u2010resolution anoscopy remains the diagnostic standard, limited access has increased interest in alternative screening. HPV testing now complements or replaces cytology in cervical cancer prevention, but evidence for anal screening, especially using extended genotyping platforms like BD Onclarity, remains limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This single center cross\u2010sectional study enrolled high\u2010risk individuals undergoing anal cancer screening from May 2024 to May 2025. Anal cytology and high\u2010risk HPV (HR\u2010HPV) DNA testing were performed using the BD Onclarity assay on the BD COR system. Demographics, cytology results, HRHPV genotypes, and available histopathology were analyzed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among 117 high\u2010risk participants (median age 42; 106 males, 8 females, 3 transgender women), 74.3% were \u2010HIV positive, predominantly MSM\u2010. Abnormal cytology occurred in 41% of cases including ASC\u2010US (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200929; 60.4%), LSIL (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200916; 33.3%), ASC\u2010H (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20092; 4.1%), and HSIL (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091; 2.1%). Valid BD Onclarity results were available for 104 individuals, with 61.5% testing HR\u2010HPV positive. Non\u201016\/18 HR\u2010HPV types were most common overall, while HPV16 predominated in abnormal cytology cases. Twelve individuals underwent biopsy, revealing no dysplasia (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20092), AIN1 (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20095), AIN2 (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091), and AIN3 (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20094).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Extended HR\u2010HPV genotyping using the BD Onclarity assay demonstrated a high prevalence of HR\u2010HPV in this high\u2010risk population, with multiple genotype infections commonly observed. While non\u2010HPV16\/18 genotypes predominated overall, HPV16 was more frequently associated with abnormal cytologic and high\u2010grade histologic findings. Given the limited number of biopsy\u2010confirmed high\u2010grade lesions, these associations should be interpreted as descriptive and hypothesis\u2010generating. Cytology remains central to anal cancer screening, with extended HPV genotyping serving as a complementary adjunct rather than a standalone stratification tool. Larger longitudinal studies with systematic histopathologic follow\u2010up are needed to clarify the clinical significance of extended HPV genotyping in this population.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dc.70160","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T14:01:15Z","timestamp":1781704875000},"page":"724-730","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extended High\u2010Risk\n                    <scp>HPV<\/scp>\n                    Genotyping With\n                    <scp>BD<\/scp>\n                    Onclarity Enhances Anal Cancer Screening Among High\u2010Risk Populations: A Cross\u2010Sectional Study"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8113-243X","authenticated-orcid":false,"given":"Daisy","family":"Maharjan","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6122-6373","authenticated-orcid":false,"given":"Joshua","family":"Waters","sequence":"additional","affiliation":[{"name":"Indiana University Health General Surgery\u2013Downtown Indianapolis  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4235-8185","authenticated-orcid":false,"given":"Melissa","family":"Randolph","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5742-7771","authenticated-orcid":false,"given":"Benjamin","family":"Shuler","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Borum","sequence":"additional","affiliation":[{"name":"Indiana University Health LifeCare  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1343-7063","authenticated-orcid":false,"given":"Sheila","family":"Segura","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,17]]},"reference":[{"key":"e_1_2_12_2_1","unstructured":"https:\/\/seer.cancer.gov\/statfacts\/html\/anus.html."},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djad128"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djad176"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir1012"},{"key":"e_1_2_12_6_1","unstructured":"\u201cRisk Factors for Anal Cancer | HPV and Anal Cancer \u201dhttps:\/\/www.cancer.org\/cancer\/types\/anal\u2010cancer\/causes\u2010risks\u2010prevention\/risk\u2010factors.html."},{"key":"e_1_2_12_7_1","unstructured":"\u201cCervical Cancer Stages\u2013NCI \u201dhttps:\/\/www.cancer.gov\/types\/cervical\/stages."},{"key":"e_1_2_12_8_1","unstructured":"\u201cUpdated Cervical Cancer Screening Guidelines | ACOG 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