{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:54:46Z","timestamp":1790999686224,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The emergence of personalized therapy and molecular sequencing has significantly transformed the management of advanced non\u2013small cell lung carcinoma (NSCLC). Supernatants obtained from fine needle aspiration (FNA) have been shown to be a reliable source of high\u2010quality nucleic acid for molecular testing. One pre\u2010analytic factor that may influence the success of molecular analysis is the collection medium used for the FNA rinse.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This prospective study evaluated 31 FNA specimens collected in three different media\u2014CytoRich Red, 10% neutral buffered formalin, or 10% bovine serum albumin (BSA)\u2014to assess their impact on DNA yield and real\u2010time polymerase chain reaction (RT\u2010PCR) outcomes for\n                      <jats:italic>KRAS (<\/jats:italic>\n                      Kirsten rat sarcoma viral oncogene homolog) and\/or\n                      <jats:italic>EGFR<\/jats:italic>\n                      (epidermal growth factor receptor) mutations. DNA extraction was performed using the QIAamp DNA FFPE (formalin\u2010fixed paraffin\u2010embedded) Tissue Kit (Qiagen), and RT\u2010PCR was conducted using the QIAGEN therascreen\n                      <jats:italic>EGFR<\/jats:italic>\n                      or\n                      <jats:italic>KRAS<\/jats:italic>\n                      RGQ PCR kits. Concurrent direct smears or formalin\u2010fixed, paraffin\u2010embedded tissue from a cell block or core biopsy served as standard controls.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the CytoRich Red group, 11 specimens (9 adenocarcinomas and 2 NSCLC) were analyzed. All 11 specimens generated results concordant with standard controls. One specimen required repeat DNA extraction due to initial pre\u2010test quality control failure. The formalin group included 12 specimens (7 squamous cell carcinomas [SCC], 3 adenocarcinomas, 1 NSCLC, and 1 large cell neuroendocrine carcinoma). Nine specimens produced concordant results with controls. Two specimens were negative for\n                      <jats:italic>EGFR<\/jats:italic>\n                      mutations, although the corresponding control tissues were not tested. Six required repeat DNA extraction because of initial pre\u2010test failure, and one specimen remained invalid after re\u2010extraction. Eight specimens were collected in BSA (5 adenocarcinomas, 2 NSCLC, and 1 SCC). Five produced concordant results with standard controls, while three specimens failed PCR pre\u2010tests despite repeat DNA extraction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In this small cohort, CytoRich Red demonstrated reliable performance for RT\u2010PCR\u2010based mutation testing. FNA supernatant represents a practical alternative specimen for lung cancer molecular analysis and may optimize specimen utilization while preserving cell block material.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dc.70184","type":"journal-article","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T06:30:40Z","timestamp":1784269840000},"page":"805-811","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Utility of Fine Needle Aspiration Supernatant for Real\u2010Time\n                    <scp>PCR<\/scp>\n                    \u2013Based Mutation Analysis in Non\u2013Small Cell Lung Cancer"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3620-7509","authenticated-orcid":false,"given":"Adeyinka","family":"Akinsanya","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University, School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2067-1162","authenticated-orcid":false,"given":"Riley","family":"Gorden","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University, School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madison","family":"Rector","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University, School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2372-2617","authenticated-orcid":false,"given":"Hamna","family":"Muhammad","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University, School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4235-8185","authenticated-orcid":false,"given":"Melissa","family":"Randolph","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University, School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt","family":"Sperling","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University, School of Medicine  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8774-0487","authenticated-orcid":false,"given":"Kemin","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University, School of Medicine  Indianapolis Indiana 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