{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:53:48Z","timestamp":1791294828405,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diagnostic Cytopathology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      p53 immunohistochemistry serves as a surrogate for\n                      <jats:italic>TP53<\/jats:italic>\n                      mutation status and plays a central role in the classification of gynecologic malignancies. However, its reliability in cytology specimens, which often constitute the initial diagnostic material, remains incompletely characterized in routine practice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      After institutional review board (IRB) approval, we performed a retrospective study of 51 gynecologic malignancies and borderline tumors with paired cytology (body fluids and fine\u2010needle aspiration specimens) and surgical resections. p53 staining patterns were classified as wild\u2010type or aberrant (overexpression, null, cytoplasmic). Concordance between specimen types and correlation with\n                      <jats:italic>TP53<\/jats:italic>\n                      mutational status were assessed. Cytology specimens were fixed in CytoRich Red fixative, while surgical specimens were formalin\u2010fixed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Overall concordance between cytology and surgical specimens was 100% (51\/51 cases), spanning multiple specimen types and tumor categories. Aberrant p53 expression was most commonly characterized by overexpression (62.8%), followed by a null pattern (23.5%). Aberrant p53 expression corresponded with\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations in all cases with available molecular data. Cytology established the initial diagnosis in 60.8% of cases, highlighting its role in early classification.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      p53 immunohistochemistry demonstrates highly reproducible, pattern\u2010based interpretation in cytology specimens, supporting its use as a reliable surrogate for\n                      <jats:italic>TP53<\/jats:italic>\n                      status at the time of initial diagnosis. These findings reinforce the role of p53 in enabling early and accurate classification of gynecologic malignancies when surgical material is limited or unavailable.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dc.70217","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:07:22Z","timestamp":1791292042000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High Concordance of p53 Immunohistochemistry Between Cytology and Surgical Specimens Supports Early Classification of Gynecologic Malignancies and Borderline Tumors"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6550-5440","authenticated-orcid":false,"given":"Devon C.","family":"Rotramel","sequence":"first","affiliation":[{"name":"Department of Pathology &amp; Laboratory Medicine Indiana University  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5930-4365","authenticated-orcid":false,"given":"Tieying","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Laboratory Medicine Indiana University  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8427-1347","authenticated-orcid":false,"given":"Harvey M.","family":"Cramer","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Laboratory Medicine Indiana University  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4584-9222","authenticated-orcid":false,"given":"Omer","family":"Saeed","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Laboratory Medicine Indiana University  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1343-7063","authenticated-orcid":false,"given":"Sheila E.","family":"Segura","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Laboratory Medicine Indiana University  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"issue":"10","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1038\/s41379-022-01102-x","article-title":"p53 Immunohistochemistry in Endometrial Cancer: Clinical and Molecular Correlates in the PORTEC\u20103 Trial","volume":"35","author":"Vermij L.","year":"2022","journal-title":"Modern Pathology"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2011.85"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqaa028"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath\u20102017\u2010204787"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep26191"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers17010038"},{"key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"155595","DOI":"10.1016\/j.prp.2024.155595","article-title":"p53 Immunohistochemistry Staining Patterns and Prognosis Significance in 212 Cases of Non\u2010Endometrioid Endometrial Cancer","volume":"263","author":"Jia H.","year":"2024","journal-title":"Pathology, Research and Practice"},{"issue":"3","key":"e_1_2_10_9_1","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.ygyno.2009.11.018","article-title":"p53 Alteration Independently Predicts Poor Outcomes in Patients With Endometrial Cancer: A Clinicopathologic Study of 131 Cases and Literature Review","volume":"116","author":"Lee E. J.","year":"2010","journal-title":"Gynecologic Oncology"},{"issue":"6","key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1111\/his.15029","article-title":"Subclonal p53 Immunostaining in the Diagnosis of Endometrial Carcinoma Molecular Subtype","volume":"83","author":"Huvila J.","year":"2023","journal-title":"Histopathology"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00192926"},{"issue":"1","key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1002\/1097-0142(19950701)76:1<72::AID-CNCR2820760110>3.0.CO;2-3","article-title":"Mutation and Allelic Loss of the p53 Gene in Endometrial Carcinoma: Incidence and Outcome in 92 Surgical Patients","volume":"76","author":"Kihana T.","year":"1995","journal-title":"Cancer"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12094\u2010025\u201004076\u20109"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cjp2.311"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20215210"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4103\/2278\u2010330X.103711"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201082206\u2010z"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010020\u201000648\u2010y"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.70029"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.5375"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0000000000000488"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22256"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.70217","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dc.70217","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.70217","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:07:23Z","timestamp":1791292043000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.70217"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,6]]},"references-count":21,"alternative-id":["10.1002\/dc.70217"],"URL":"https:\/\/doi.org\/10.1002\/dc.70217","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,6]]},"assertion":[{"value":"2026-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"dc.70217"}}