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Berberine (BBR) has various confirmed biological activities, such as anti\u2010inflammatory and antioxidant activities. However, the effect of BBR on the production of inflammation\u2010associated proteins, including inducible nitric oxide synthase (iNOS), cyclooxygenase (Cox)\u20102, metalloproteinases (MMPs), Collagen II, TNF\u2010\u03b1, and IL\u20106 via the MAPK (mitogen\u2010activated protein kinases) pathway in IL\u20101\u03b2\u2010stimulated rat chondrocytes, has not yet been studied. Thus, the purpose of this study was to evaluate whether BBR would decrease the production of inflammation\u2010associated proteins through the MAPK signal pathway. Rat chondrocytes were cultured and pretreated with BBR at different concentrations (0, 25, 50, and 100\u2009\u03bcM) and then stimulated with or without IL\u20101\u03b2 (10 ng\/mL). The mRNA expression of iNOS, COX\u20102, MMP\u20103, MMP\u201013, TNF\u2010\u03b1, and IL\u20106 was measured by real\u2010time polymerase chain reaction (RT\u2010PCR), and the protein expression of iNOS, COX\u20102, Collagen II, MMP\u20103,MMP\u201013, and MAPKs were measured by Western blotting. The results showed that the expression of iNOS, COX\u20102, MMP\u20103, MMP\u201013, TNF\u2010\u03b1, and IL\u20106 increased in the IL\u20101\u03b2\u2010treated group and BBR showed an ability to inhibit the elevated expression under the pretreatment. Furthermore, the IL\u20101\u03b2\u2010induced downregulation of Collagen II could be ameliorated by BBR. Moreover, the expression of MAPKs was significantly decreased by BBR. These results demonstrated that BBR had the anti\u2010catabolic and anti\u2010inflammation abilities that were through the MAPKs in IL\u20101\u03b2\u2010induced rat chondrocytes. These findings may provide a novel therapeutic choice for treatment of OA using BBR.<\/jats:p>","DOI":"10.1002\/ddr.21541","type":"journal-article","created":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T07:01:56Z","timestamp":1556521316000},"page":"637-645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Berberine inhibits the interleukin\u20101 beta\u2010induced inflammatory response via MAPK downregulation in rat articular chondrocytes"],"prefix":"10.1002","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5676-8962","authenticated-orcid":false,"given":"Xing","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery The Second Affiliated Hospital of Guangzhou University of Chinese Medicine  No. 111 Dade Road, Guangzhou Guangdong China"},{"name":"Department of Orthopedic Surgery, Lingnan Medical Research Center of Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Guangzhou University of Chinese Medicine  No. 12, Jichang Road, Baiyun District, Guangzhou P. 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