{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T23:17:21Z","timestamp":1769555841849,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,7,13]],"date-time":"2019-07-13T00:00:00Z","timestamp":1562976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/135871\/2018"],"award-info":[{"award-number":["SFRH\/BD\/135871\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/AGR\/04033\/2019"],"award-info":[{"award-number":["UID\/AGR\/04033\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/EQU\/00511\/2019"],"award-info":[{"award-number":["UID\/EQU\/00511\/2019"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Development Research"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The nuclear factor kappa light chain enhancer of activated B cells (NF\u2010\u03baB) has been implicated in the progression of cancers induced by high\u2010risk human papillomaviruses (HPV). In cancer patients, NF\u2010\u03baB is also thought to drive a chronic systemic inflammatory status, leading to cachexia. This study addressed the ability of dimethylaminoparthenolide (DMAPT), a water\u2010soluble NF\u2010\u03baB inhibitor, to block the development of HPV\u2010induced lesions and wasting syndrome in HPV16\u2010transgenic mice. Mice received DMAPT orally (100\u2009mg\/kg\/day), once a day, for 6 consecutive weeks. Body weight was monitored weekly along with food and water intake. After 6 weeks the animals were submitted to a grip strength test and sacrificed for specimen collection. Skin samples were analyzed histologically and for expression of NF\u2010\u03baB\u2010regulated genes <jats:italic>Bcl2<\/jats:italic> and <jats:italic>Bcl2l1<\/jats:italic>. Gastrocnemius muscles were weighted and analyzed for expression of NF\u2010\u03baB subunits p50, p52, p65, and Rel\u2010B. DMAPT reduced the incidence of epidermal dysplasia (18.2% versus 33.3% in HPV16<jats:sup>+\/\u2212<\/jats:sup> untreated mice). This was associated with reduced expression of <jats:italic>Bcl2<\/jats:italic> and <jats:italic>Bcl2l1<\/jats:italic> (<jats:italic>p<\/jats:italic> = .0003 and <jats:italic>p<\/jats:italic> = .0014, respectively) and reduced neutrophilic infiltration (<jats:italic>p<\/jats:italic> = .0339). Treated mice also showed partially preserved bodyweight and strength, which were independent of the expression levels of NF\u2010\u03baB subunits in skeletal muscle.These results suggest that NF\u2010\u03baB inhibition may be a valid strategy against HPV\u2010induced lesions in vivo and warrant further preclinical tests particularly in the set of combination therapies. In addition, the data may support the use of DMAPT to prevent wasting syndrome.<\/jats:p>","DOI":"10.1002\/ddr.21565","type":"journal-article","created":{"date-parts":[[2019,7,13]],"date-time":"2019-07-13T10:19:56Z","timestamp":1563013196000},"page":"824-830","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Dimethylaminoparthenolide reduces the incidence of dysplasia and ameliorates a wasting syndrome in HPV16\u2010transgenic mice"],"prefix":"10.1002","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6268-524X","authenticated-orcid":false,"given":"Joana M. O.","family":"Santos","sequence":"first","affiliation":[{"name":"Molecular Oncology and Viral Pathology Group, IPO Porto Research Center (CI\u2010IPOP) Portuguese Oncology Institute of Porto (IPO Porto)  Porto Portugal"},{"name":"Faculty of Medicine of the University of Porto (FMUP)  Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Moreira\u2010Pais","sequence":"additional","affiliation":[{"name":"Organic Chemistry, Natural and Agro\u2010Food Products Research Unit (QOPNA) University of Aveiro (UA)  Aveiro Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiago","family":"Neto","sequence":"additional","affiliation":[{"name":"Molecular Oncology and Viral Pathology Group, IPO Porto Research Center (CI\u2010IPOP) Portuguese Oncology Institute of Porto (IPO Porto)  Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Peixoto da Silva","sequence":"additional","affiliation":[{"name":"Molecular Oncology and Viral Pathology Group, IPO Porto Research Center (CI\u2010IPOP) Portuguese Oncology Institute of Porto (IPO Porto)  Porto Portugal"},{"name":"Faculty of Medicine of the University of Porto (FMUP)  Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula A.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centre for the Research and Technology of Agro\u2010Environmental and Biological Sciences (CITAB) University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro (UTAD)  Vila Real Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Organic Chemistry, Natural and Agro\u2010Food Products Research Unit (QOPNA) University of Aveiro (UA)  Aveiro Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaquim","family":"Mendes","sequence":"additional","affiliation":[{"name":"Institute of Science and Innovation in Mechanical and Industrial Engineering (INEGI), Faculty of Engineering of the University of Porto (FEUP)  Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarida M. S. M.","family":"Bastos","sequence":"additional","affiliation":[{"name":"Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE) Faculty of Engineering of the University of Porto (FEUP)  Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Lopes","sequence":"additional","affiliation":[{"name":"Botelho Moniz An\u00e1lises Cl\u00ednicas (BMAC)  Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1tima","family":"Casaca","sequence":"additional","affiliation":[{"name":"Botelho Moniz An\u00e1lises Cl\u00ednicas (BMAC)  Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Silva","sequence":"additional","affiliation":[{"name":"Botelho Moniz An\u00e1lises Cl\u00ednicas (BMAC)  Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Sweeney","sequence":"additional","affiliation":[{"name":"Dana\u2010Farber Cancer Institute  Boston Massachusetts"},{"name":"Harvard Medical School  Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3010-8373","authenticated-orcid":false,"given":"Rui","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Molecular Oncology and Viral Pathology Group, IPO Porto Research Center (CI\u2010IPOP) Portuguese Oncology Institute of Porto (IPO Porto)  Porto Portugal"},{"name":"Faculty of Medicine of the University of Porto (FMUP)  Porto Portugal"},{"name":"Virology Service, Portuguese Oncology Institute of Porto (IPO Porto)  Porto Portugal"},{"name":"Research Department of the Portuguese League Against Cancer\u2014Regional Nucleus of the North (Liga Portuguesa Contra o Cancro\u2014N\u00facleo Regional do Norte)  Porto Portugal"},{"name":"Biomedical Research Center (CEBIMED) Faculty of Health Sciences of the Fernando Pessoa University  Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui M.","family":"Gil da Costa","sequence":"additional","affiliation":[{"name":"Molecular Oncology and Viral Pathology Group, IPO Porto Research Center (CI\u2010IPOP) Portuguese Oncology Institute of Porto (IPO Porto)  Porto Portugal"},{"name":"Centre for the Research and Technology of Agro\u2010Environmental and Biological Sciences (CITAB) University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro (UTAD)  Vila Real Portugal"},{"name":"Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE) Faculty of Engineering of the University of Porto (FEUP)  Porto Portugal"},{"name":"Postgraduate Programme in Adult Health (PPGSAD) and Tumour Biobank Federal University of Maranh\u00e3o (UFMA)  S\u00e3o Lu\u00eds Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2014.911919"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/10408444.2013.813905"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0496"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4251\/wjgo.v7.i4.17"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.7.4358-4368.1994"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204926"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.8.2687-2695.2000"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0168065"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2005.04.014"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-017-0008-3"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.09.029"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2015.2884"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2004.01.024"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2016.10.031"},{"issue":"5","key":"e_1_2_8_16_1","first-page":"2073","article-title":"The NFkappaB signaling pathway in papillomavirus\u2010induced lesions: Friend or foe?","volume":"36","author":"Gil da Costa R. M.","year":"2016","journal-title":"Anticancer Research"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2013.00120"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-05-090621"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR1128.1"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR14710.1"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2012.05.009"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.21873\/anticanres.12371"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2009.05.092"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2014.12.026"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1593\/neo.07871"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116868"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2015.02.020"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3204"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/IGC.0b013e3181e64bb1"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2009.10.008"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2014.90"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2016.05.040"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.21141"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-7-research0034"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-17-0717"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2006.9.55"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00538-10"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21167"}],"container-title":["Drug Development Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fddr.21565","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ddr.21565","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ddr.21565","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ddr.21565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T21:21:20Z","timestamp":1760390480000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/ddr.21565"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,13]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1002\/ddr.21565"],"URL":"https:\/\/doi.org\/10.1002\/ddr.21565","archive":["Portico"],"relation":{},"ISSN":["0272-4391","1098-2299"],"issn-type":[{"value":"0272-4391","type":"print"},{"value":"1098-2299","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,13]]},"assertion":[{"value":"2019-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}